文献类型：期刊文献

英文题名：Laboratory and field evaluation of the transgenic Populus alba x Populus glandulosa expressing double coleopteran-resistance genes

作者：Zhang, Bingyu[1] Chen, Min[2] Zhang, Xiaofen[2] Luan, Hehui[1] Diao, Shu[1] Tian, Yingchuan[3] Su, Xiaohua[1]

第一作者：张冰玉

通信作者：Su, XH[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, Key Lab Tree Breeding & Cultivat, Beijing 100091, Peoples R China;[2]Beijing Forestry Univ, Key Lab Silviculture & Conservat, Minist Educ, Beijing 100082, Peoples R China;[3]Chinese Acad Sci, Inst Microbiol, Beijing 100080, Peoples R China

年份：2011

卷号：31

期号：5

起止页码：567-573

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000291893600011)】；

基金：This work was supported by the Special Fund for Forestry-Scientific Research in the Public Interest (201004004), the National High-Tech R&D Program (2009AA10Z107) and the Key Program of Genetic Engineering of New Organisms of China (2009ZX08009-098B).

语种：英文

外文关键词：Bt-Cry3A; field evaluation; insect bioassay; OC-I; transgenic poplar

摘要：Expression of the two coleopteran-resistant proteins (Bt-Cry3A and oryzacystatin I) was detected in the leaves of field-grown transgenic poplar (BOGA-5) in two or three subsequent years. The BOGA-5 contained similar to 10 mu g g(-1) of Cry3A over the individual years with no detection in the control, and protein extracts from BOGA-5 displayed a higher reduction in papain activity (similar to 42%) compared with similar to 21% in the control. Laboratory feeding experiments showed that the total mortality of the target pest Plagiodera versicolora (Coleoptera, Chrysomelida) larvae fed with BOGA-5 leaves was 76.7%, significantly higher than that of the control (P < 0.05). However, no significant differences were detected in the mortality, exuviation index, pupation rate or adult eclosion rate of the non-target Clostera anachoreta (Lepidoptera, Notodontidae) fed with leaves from transgenic and non-transgenic poplars. Field investigation indicated that the transgenic poplar retained coleopteran insect resistance in the field, suggesting the potential use of the double gene transgenic poplar for pest management in commercial poplar plantations.