文献类型：期刊文献

英文题名：Long-term nitrogen and phosphorus fertilization reveals that phosphorus limitation shapes the microbial community composition and functions in tropical montane forest soil

作者：Ma, Xiaomin[1] Zhou, Zhang[2] Chen, Jie[2] Xu, Han[2] Ma, Suhui[3] Dippold, Michaela A.[4] Kuzyakov, Yakov[5,6]

第一作者：Ma, Xiaomin

通信作者：Chen, J[1]

机构：[1]Zhejiang A&F Univ, State Key Lab Subtrop Silviculture, Hangzhou 311300, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[3]Peking Univ, Key Lab Earth Surface Proc, Coll Urban & Environm Sci, Inst Ecol,Minist Educ, Beijing, Peoples R China;[4]Univ Tubingen, Geobiosphere Interact, Tuebingen Schnarrenberg Str 94-96, D-72076 Tubingen, Germany;[5]Univ Goettingen, Dept Agr Soil Sci, Dept Soil Sci Temperate Ecosyst, D-37077 Gottingen, Germany;[6]Peoples Friendship Univ Russia RUDN Univ, Moscow 117198, Russia

年份：2023

卷号：854

外文期刊名：SCIENCE OF THE TOTAL ENVIRONMENT

收录：;EI(收录号：20223912789669);Scopus(收录号：2-s2.0-85138385845);WOS:【SCI-EXPANDED(收录号:WOS:000862758300017)】；

基金：Acknowledgements This study was supported by the National Natural Science Foundation of China (31901161, 32001170, and 31872701) , the Science and Technology Planning Project of Guangzhou (202102021182) and the RUDN University Strategic Academic Leadership Program.

语种：英文

外文关键词：Nitrogen and phosphorus fertilization; Microbial community composition; Enzyme activity; Functional gene abundance; Nutrient cycling; Tropical montane forest

摘要：Microorganisms govern soil nutrient cycling. It is therefore critical to understand their responses to human-induced increases in N and P inputs. We investigated microbial community composition, biomass, functional gene abundance, and enzyme activities in response to 10-year N and P addition in a primary tropical montane forest, and we explored the drivers behind these effects. Fungi were more sensitive to nutrient addition than bacteria, and the fungal commu-nity shift was mainly driven by P availability. N addition aggravated P limitation, to which microbes responded by in-creasing the abundance of P cycling functional genes and phosphatase activity. In contrast, P addition alleviated P deficiency, and thus P cycling functional gene abundance and phosphatase activity decreased. The shift of microbial community composition, changes in functional genes involved in P cycling, and phosphatase activity were mainly driven by P addition, which also induced the alteration of soil stoichiometry (C/P and N/P). Eliminating P deficiency through fertilization accelerated C cycling by increasing the activity of C degradation enzymes. The abundances of C and P functional genes were positively correlated, indicating the intensive coupling of C and P cycling in P-limited for-est soil. In summary, a long-term fertilization experiment demonstrated that soil microorganisms could adapt to in-duced environmental changes in soil nutrient stoichiometry, not only through shifts of microbial community composition and functional gene abundances, but also through the regulation of enzyme production. The response of the microbial community to N and P imbalance and effects of the microbial community on soil nutrient cycling should be incorporated into the ecosystem biogeochemical model.