文献类型：期刊文献

英文题名：Moderate NO3--N instead of NH4+-N alleviates the effect of salt stress on walnut growth

作者：Su, Gaoya[1] Lu, Sen[1] Bai, Yongchao[1] Pei, Dong[1] Wang, Yan[2] Deng, Hao[3]

第一作者：Su, Gaoya

通信作者：Lu, S[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, 1 Dong Xiao Fu, Beijing 100091, Peoples R China;[2]Xinjiang Acad Forestry Sci, Pomol Res Base Jiamu, Wensu 843100, Peoples R China;[3]Xinjiang Inst Technol, Akesu 843000, Peoples R China

年份：2025

卷号：12

期号：1

外文期刊名：CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE

收录：;Scopus(收录号：2-s2.0-105021425539);WOS:【SCI-EXPANDED(收录号:WOS:001615510800001)】；

基金：This work was supported by the Xinjiang "Jie Bang Gua Shuai" Research Project (2023-10), the National Key R&D Program of China (2022YFD1000100), the "Tian Chi Ying Cai" Project, and the Natural Science Foundation of China (41877019).

语种：英文

外文关键词：Salt stress; NO3-/NH4+ nitrogen fertilizer; Plant performance; Rhizosphere microbiome; Juglans regia L.

摘要：Walnut (Juglans regia L.) is an important oilseed crop, and salt stress threatens the growth of walnut tree. In this study, NO3- or NH4+ was applied at three concentrations (4, 32, and 100 mM) to investigate the effect of NO3--N and NH4+-N on walnut seedlings under 100 mM NaCl stress. Results showed that moderate (32 mM) NO3--N application alleviated the effect of salt stress. Moreover, plants treated with 32 mM NO3--N showed no significant morphological difference from those subjected to the nonstress treatment. The treatment of 32 mM NO3--N application enhanced plant growth, increased antioxidant enzyme activities (superoxide dismutase and catalase), and restricted Na+ and Cl- uptake and transport. Additionally, it might induce beneficial shifts in the rhizosphere microbiome. Low-concentration (4 mM) NO3- or NH4+ treatment individually induced the minor alleviation of salt stress. By contrast, 100 mM NO3- and all tested concentrations of NH4+ further inhibited biomass and root growth, thereby exacerbating salt injury. Notably, 100 mM NH4+ caused severe defoliation and seedling mortality. Furthermore, in contrast to its NO3- counterpart, 32 mM NH4+ shifted the root microbiome and impaired microbial diversity, likely contributing to increased salt sensitivity. This study demonstrates that moderate NO3- application can effectively mitigate salt stress during walnut growth, offering a potential strategy for fertilizing walnut plantations under saline conditions. [GRAPHICS]