文献类型：期刊文献

英文题名：Genetic identification of 91 poplar cultivars based on SSR markers

作者：Sun, Pei[1] Jia, Huixia[1] Yue, Zhiqiang[1] Li, Jinhua[1] Li, Jianbo[1] Zhang, Jin[1,2] Lu, Mengzhu[1,3] Hu, Jianjun[1,3]

通信作者：Hu, JJ[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat, Res Inst Forestry,Natl Forestry & Grassland Adm, Xiangshan St, Beijing, Peoples R China;[2]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN USA;[3]Nanjing Forestry Univ, Collaborat Innovat Ctr Sustainable Forestry South, Nanjing, Peoples R China

年份：2020

卷号：38

期号：6

外文期刊名：NORDIC JOURNAL OF BOTANY

收录：;Scopus(收录号：2-s2.0-85087703504);WOS:【SCI-EXPANDED(收录号:WOS:000551935600005)】；

基金：This research was funded by the National Nonprofit Institute Research Grant of the Chinese Academy of Forestry, grant no. CAFYBB2017ZY008 and CAFYBB2018ZY001-9, and the National Natural Science Foundation of China, grant no. 31570669.

语种：英文

外文关键词：fingerprint; flow cytometry; Populus; triploid; UPGMA analysis

摘要：Accurate genetic identification and relationship analysis of poplar cultivars is necessary to establish commercial poplar plantations and select suitable breeding strategies. In this study, 91 poplar cultivars belonging to four sections (Aigeiros,Tacamahaca,PopulusandTuranga) and inter/intra-sectional hybrids were genotyped using 18 polymorphic simple sequence repeat (SSR) markers. In total, 222 alleles were amplified with an average of 12.3 alleles per marker. The mean polymorphic information content and power of discrimination were 0.706 and 0.813, respectively. Five SSR markers (ORPM_103, ORPM_247, GCPM_1048, GCPM_1255 and LG_X_19) constituted a core fingerprint and were sufficient to identify all the tested cultivars. With some notable exceptions, cultivars of the same species generally clustered together in cluster (UPGMA) and ordination (PCO) analyses. Flow cytometry indicated that 11 poplar cultivars were triploid. Among these, seven had three alleles at some loci, suggesting that SSR markers could indicate the ploidy level to some extent. This study provides useful genetic information for the identification and protection of poplar cultivars in China and offers a guideline for the selection of poplar crossing parents based on ploidy level and genetic relationships.