文献类型：期刊文献

英文题名：Phytohalomonas tamaricis gen. nov., sp. nov., an endophytic bacterium isolated from Tamarix ramosissima roots growing in Kumtag desert

作者：Liu, Lei[1] Wang, Shi-yan[1] He, Cong-fen[2] Zhang, Xiao-xia[3] Chi, Miao[4] Liang, Li-xiong[1] Li, Lu-bin[1]

第一作者：刘蕾

通信作者：Li, LB[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat,State Forestry A, Beijing 100091, Peoples R China;[2]Beijing Technol & Business Univ, Beijing Key Lab Plant Resource Res & Dev, Beijing 100048, Peoples R China;[3]Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Microbial Resources Collect & Preservat, Minist Agr, Beijing 100081, Peoples R China;[4]Beijing Bot Garden, Beijing 100093, Peoples R China

年份：2020

卷号：202

期号：1

起止页码：143-151

外文期刊名：ARCHIVES OF MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000511494200014)】；

基金：This research was funded by a grant from the Opening Foundation of Beijing Key Laboratory of Plant Resources Research and Development (PRRD-2016-ZD1). This research was supported by The National Key Technologies R&D Program of China (2016YFC050080104).

语种：英文

外文关键词：Halomonadaceae; Phytohalomonas tamaricis; Tamarix ramosissima; Endophytic bacterium; Kumtag desert

摘要：A gram-stain-negative, aerobic, non-spore-forming, rod-shaped, non-motile bacterium strain R4HLG17(T) was isolated from Tamarix ramosissima roots growing in Kumtag desert. The strain grew at salinities of 0-16% (w/v) NaCl (optimum 5-6%), pH 5-9 (optimum 7) and at 16-45 degrees C. Based on 16S rRNA gene sequence similarity, strain R4HLG17(T) belonged to the family Halomonadaceae and was most closely related to Halomonas lutea DSM 23508(T)(95.1%), followed by Halotalea alkalilenta AW-7(T)(94.8%), Salinicola acroporae S4-41(T)(94.8%), Salinicola halophilus CG4.1(T)(94.6%), and Larsenimonas salina M1-18(T)(94.4%). Multilocus sequence analysis (MLSA) based on the partial sequences of 16S rRNA, atpA, gyrB, rpoD, and secA genes indicated that the strain R4HLG17(T) formed an independent and monophyletic branch related to other genera of Halomonadaceae, supporting its placement as a new genus in this family. The draft genome of strain R4HLG17(T) was 3.6 Mb with a total G + C content of 55.1%. The average nucleotide identity to Halomonas lutea DSM 23508(T) was 83.5%. Q-9 was detected as the major respiratory quinone and summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c), summed feature 3 (C-16:1 omega 7c/C-16:1 omega 6c), and C-16:0 as predominant cellular fatty acids. On the basis of chemotaxonomic, phylogenetic, and phenotypic evidence, strain R4HLG17(T) is concluded to represent a novel species of a new genus within Halomonadaceae, for which the name Phytohalomonas tamaricis gen. nov., sp. nov., is proposed. The type strain is R4HLG17(T) (=ACCC 19929(T)=KCTC 52415(T)).