文献类型：期刊文献

中文题名：华南红树林溶磷菌16S rDNA PCR-RFLP分析及其溶磷能力

英文题名：Analysis of the 16S rDNA PCR-RFLP and the Phosphate-Dissolving Capacity of Phosphate-Dissolving Bacteria Isolated from Rhizosphere of Mangrove in Southern China

作者：陆俊锟[1] 陈俊[1,2] 康丽华[1] 杨振德[2]

第一作者：陆俊锟

机构：[1]中国林业科学研究院热带林业研究所,广州510520;[2]广西大学林学院,南宁530004

年份：2009

卷号：45

期号：5

起止页码：137-142

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2008】；CSCD:【CSCD2011_2012】；

基金：国家自然科学基金项目(30671668)

语种：中文

中文关键词：红树林;溶磷菌;RFLP;系统发育;多样性;溶磷能力

外文关键词：mangrove; phosphate-dissolving bacteria; RFLP; phylogeny; diversity; phosphate-dissolving capacity

分类号：Q933

摘要：Phosphate-dissolving bacteria isolated from rhizosphere of mangrove were characterized by 16S rDNA PCR-RFLP and 16S rDNA sequence to describe the genetic diversity and phylogeny. The dendrogram of 16S rDNA PCR-RFLP revealed the 24 isolated stains were assembled at 59% similarity level. The strains were grouped into 7 subgroups at 80% similarity. Phylogenetic tree of 16S rDNA sequences was constructed with representative strains in reference to the homogenous sequences in Genebank,and the result showed that Bacillus sp. was the dominant member,besides Paenibacillus sp.,Vibrio sp.,Klebsiella sp. and Pseudomonas sp. were also found.The highly genetic diversity was highly revealed by this study.In addition,the phosphate-dissolving capacity to dissolve solubilize the unsolvable inorganic phosphorus of bacteria was tested.All representative strains had the phosphate-dissolving capacity.The pH of liquid media of strains both were reduced to some degree,but the correlations were not found between phosphate-dissolving capacity and pH of the culture solution.
Phosphate-dissolving bacteria isolated from rhizospbere of mangrove were characterized by 16S rDNA PCR-RFLP and 16S rDNA sequence to describe the genetic diversity and phylogeny. The dendrogram of 16S rDNA PCR-RFLP revealed the 24 isolated stains were assembled at 59% similarity level. The strains were grouped into 7 subgroups at 80% similarity. Phylogenetic tree of 16S rDNA sequences was constructed with representative strains in reference to the homogenous sequences in Genebank, and the result showed that Bacillus sp. was the dominant member, besides Paenibacillus sp., Vibrio sp., Klebsiella sp. and Pseudomonas sp. were also found. The highly genetic diversity was highly revealed by this study. In addition, the phosphate-dissolving capacity to dissolve solubilize the unsolvable inorganic phosphorus of bacteria was tested. All representative strains had the phosphate-dissolving capacity. The pH of liquid media of strains both were reduced to some degree, but the correlations were not found between phosphate-dissolving capacity and pH of the culture solution.