文献类型：期刊文献

英文题名：Effect of Persistent Salt Stress on the Physiology and Anatomy of Hybrid Walnut (Juglans major x Juglans regia) Seedlings

作者：Tang, Jiali[1] Ji, Xinying[1] Li, Ao[1] Zheng, Xu[1] Zhang, Yutong[2] Zhang, Junpei[1]

第一作者：Tang, Jiali

通信作者：Zhang, JP[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]China Agr Univ, Coll Resources & Environm Sci, Beijing 100091, Peoples R China

年份：2024

卷号：13

期号：13

外文期刊名：PLANTS-BASEL

收录：;WOS:【SCI-EXPANDED(收录号:WOS:001270337900001)】；

基金：This work was supported by the National Key Research and Development Program of China (2022YFD2200402).

语种：英文

外文关键词：Juglans; salt stress; leaf and root anatomy; physiological characteristics; seedling growth

摘要：Soil salinization has become one of the major problems that threaten the ecological environment. The aim of this study is to explore the mechanism of salt tolerance of hybrid walnuts (Juglans major x Juglans regia) under long-term salt stress through the dynamic changes of growth, physiological and biochemical characteristics, and anatomical structure. Our findings indicate that (1) salt stress inhibited seedling height and ground diameter increase, and (2) with increasing salt concentration, relative water content (RWC) decreased, and proline (Pro) and soluble sugar (SS) content increased. The Pro content reached a maximum of 549.64 mu g/g on the 42nd day. The increase in superoxide dismutase (SOD) activity (46.80-117.16%), ascorbate peroxidase (APX) activity, total flavonoid content (TFC), and total phenol content (TPC) under salt stress reduced the accumulation of malondialdehyde (MDA). (3) Increasing salt concentration led to increases and subsequent decreases in the thickness of palisade tissues, spongy tissues, leaves, and leaf vascular bundle diameter. Upper and lower skin thickness, root periderm thickness, root diameter, root cortex thickness, and root vascular bundle diameter showed different patterns of change at varying stress concentrations and durations. Overall, the study concluded that salt stress enhanced the antireactive oxygen system, increased levels of osmotic regulators, and low salt concentrations promoted leaf and root anatomy, but that under long-term exposure to high salt levels, leaf anatomy was severely damaged. For the first time, this study combined the anatomical structure of the vegetative organ of hybrid walnut with physiology and biochemistry, which is of great significance for addressing the challenge of walnut salt stress and expanding the planting area.