详细信息
Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress ( SCI-EXPANDED收录) 被引量:31
文献类型:期刊文献
英文题名:Comparative proteomic analysis in Miscanthus sinensis exposed to antimony stress
作者:Xue, Liang[1,2] Ren, Huadong[1] Li, Sheng[1] Gao, Ming[1,2] Shi, Shengqing[2] Chang, Ermei[2] Wei, Yuan[2] Yao, Xiaohua[1] Jiang, Zeping[2] Liu, Jianfeng[2]
第一作者:薛亮
通信作者:Liu, JF[1]
机构:[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China
年份:2015
卷号:201
起止页码:150-160
外文期刊名:ENVIRONMENTAL POLLUTION
收录:;WOS:【SCI-EXPANDED(收录号:WOS:000353081500017)】;
基金:The work was funded by the Ministry of Science and Technology, China (2012BAC09B03 and 2011BAD38B0103).
语种:英文
外文关键词:Proteomic; Antimony; Stress; Miscanthus sinensis
摘要:To explore the molecular basis of Sb tolerance mechanism in plant, a comparative proteomic analysis of both roots and leaves in Miscanthus sinensis has been conducted in combination with physiological and biochemical analyses. M. sinensis seedlings were exposed to different doses of Sb, and both roots and leaves were collected after 3 days of treatment. Two-dimensional gel electrophoresis (2-DE) and image analyses found that 29 protein spots showed 1.5-fold change in abundance in leaves and 19 spots in roots, of which 31 were identified by MALDI-TOF-MS and MALDI-TOF-TOF-MS. Proteins involved in antioxidant defense and stress response generally increased their expression all over the Sb treatments. In addition, proteins relative to transcription, signal transduction, energy metabolism and cell division and cell structure showed a variable expression pattern over Sb concentrations. Overall these findings provide new insights into the probable survival mechanisms by which M. sinensis could be adapting to Sb phytotoxicity. (C) 2015 Elsevier Ltd. All rights reserved.
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