文献类型：期刊文献

中文题名：麻竹人工授粉群体连锁图谱的构建

英文题名：The Genetic Linkage Map Construction of Dendrocalamus latiflorus Munro

作者：袁金玲[1] 傅懋毅[1] 姜景民[1]

第一作者：袁金玲

机构：[1]中国林业科学研究院亚热带林业研究所

年份：2005

卷号：19

期号：4

起止页码：21-23

中文期刊名：林业科技开发

外文期刊名：China Forestry Science and Technology

收录：CSTPCD

基金：林业部指南项目"竹类植物遗传多样性和遗传连锁图谱的构建"(编号:190)。

语种：中文

中文关键词：麻竹;连锁图谱;RAPD;拟测交;人工授粉;构建;群体;RAPD标记;控制授粉;随机引物

外文关键词：Dendrocalamus latiflorus Munro; Linkage map; RAPD; Pseudo-testcross

分类号：S828.2;S651

摘要：利用人工控制授粉获得的麻竹F1群体,通过RAPD标记按拟测交作图策略构建了麻竹的连锁图谱。试验过程中从250个10bp的随机引物中筛选出16个,对双亲及44个子代进行扩增,共获得101条谱带,其中46条为符合1∶1分离的拟测交位点。利用多点连锁分析,共形成7个连锁群,覆盖的总图距为305.7cM,最长为100.0cM,最短为12.4cM,标记间的平均图据约为12.74cM,从而为构建高密度的麻竹遗传图谱提供了基础。
F1 generation of Dendrocalamus latiflorus Mtmro was obtained from artificial pollination, and random amplified polymorphic DNAs （RAPDs） were used to construct linkage maps of the parents of them. 16 random primers were screened out from 250 ones to generate a sample of 44 F1 progenies and their parents, total 101 loci were identified, among which 46 were segregating 1：1 （testcross configuration）.7 linkage groups were formed by using multiple analysis, the resulting linkage map of Dendrocalamus latiflorus Munro sparmed 305.7cM, with the largest of 100.0 cM and the shortest of 12.4 cM,the average distance is 12.74 cM, and this provide bases for construction a advanced genetic maps.