文献类型：期刊文献

英文题名：Metagenomics reveals the effects of glyphosate on soil microbial communities and functional profiles of C and P cycling in the competitive vegetation control process of Chinese fir plantation

作者：Wang, Song[1] Han, Yuanyuan[1] Wu, Xiaoyu[2] Sun, Honggang[1]

通信作者：Sun, HG[1]

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[2]Chinese Acad Forestry, Expt Ctr Subtrop Forestry, Fenyi 336600, Peoples R China

年份：2023

卷号：238

外文期刊名：ENVIRONMENTAL RESEARCH

收录：;EI(收录号：20233814752230);Scopus(收录号：2-s2.0-85171444945);WOS:【SCI-EXPANDED(收录号:WOS:001079456700001)】；

基金：This work was funded by the National Key Research and Develop- ment Program of China (grant no. 2021YFD2201303-03) .

语种：英文

外文关键词：Glyphosate; Chinese fir plantation; Metagenomics; Soil microbiome; Competitive vegetation management; Microbial functional gene

摘要：Although considerable efforts have been devoted to investigate the behavior of glyphosate on microbiome in various environment, knowledge about the soil microbial community and functional profile in weeds control process of the Chinese fir plantation are limited. In this study, shotgun metagenomic sequencing was used to determine the abundance and diversity of microbial communities and functional genes after foliar application of glyphosate for 1, 2, 3 and 4 months in a Chinese fir plantation. The results showed that glyphosate increased the copy numbers (qPCR) of 16S rRNA gene for 16.9%, improved the bacterial diversity (Shannon index) and complexity of bacterial co-occurrence network, and changed the abundances of some bacterial and fungal taxa, but had no effects on ITS gene copy numbers, fungal Shannon index, and bacterial and fungal communities (PCoA). Glyphosate application significantly decreased the amount of microbial function potentials involved in organic P mineralization for 10.7%, chitin degradation for 13.1%, and CAZy gene families with an exception of PL for 11.5% at the first month, while did not affect the profile of microbial genes response to P and C cycling in longer term. In addition, glyphosate reduced the contents of soil TOC, DOC and NH4+-H for 17.6%, 52.3% and 44.6% respectively, and decreased the starch, soluble sugar, Zn and Fe of Chinese fir leaves for 20.6%, 19.8%, 32.8% and 48.4% respectively. Mantle test, Spearman's correlation, and PLS-PM model revealed the connections among soil properties, tree nutrients, bacterial and fungal communities, and microbial function potentials were influenced by glyphosate. While our findings need to be validated in other filed and mechanistic studies, they may indicate that the foliar application of glyphosate has a potential effect on Chinese fir seedlings, and this effect may contribute to the changes of the bacterial community and soil properties including AN, DON and NH4+- H.