文献类型：期刊文献

英文题名：Transcriptome and structure analysis in root of Casuarina equisetifolia under NaCl treatment

作者：Wang, Yujiao[1] Zhang, Jin[2] Qiu, Zhenfei[1] Zeng, Bingshan[1] Zhang, Yong[1] Wang, Xiaoping[1] Chen, Jun[1] Zhong, Chonglu[1] Deng, Rufang[3] Fan, Chunjie[1]

第一作者：Wang, Yujiao

通信作者：Fan, CJ[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Key Lab State Forestry & Grassland Adm Trop Fores, Guangzhou, Peoples R China;[2]Zhejiang A&F Univ, Sch Forestry & Biotechnol, State Key Lab Subtrop Silviculture, Hangzhou, Zhejiang, Peoples R China;[3]Chinese Acad Sci, South China Bot Garden, Guangzhou, Peoples R China

年份：2021

卷号：9

外文期刊名：PEERJ

收录：;EI(收录号：20232314204002);Scopus(收录号：2-s2.0-85115409575);WOS:【SCI-EXPANDED(收录号:WOS:000707255200003)】；

基金：This work was supported by a grant from the Specific Program for National Non-profit Scientific Institutions (CAFYBB2018ZB003) , a project funded by the National Natural Science Foundation of China (Grant No. 31770716) . The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

语种：英文

外文关键词：Salt stress; Differentially expressed genes; Epidermal cells; Ion content; Programmed cell death

摘要：Background. High soil salinity seriously affects plant growth and development. Excessive salt ions mainly cause damage by inducing osmotic stress, ion toxicity, and oxidation stress. Casuarina equisetifolia is a highly salt-tolerant plant, commonly grown as wind belts in coastal areas with sandy soils. However, little is known about its physiology and the molecular mechanism of its response to salt stress. Results. Eight-week-old C. equisetifolia seedlings grown from rooted cuttings were exposed to salt stress for varying durations (0, 1, 6, 24, and 168 h under 200 mM NaCl) and their ion contents, cellular structure, and transcriptomes were analyzed. Potassium concentration decreased slowly between 1 h and 24 h after initiation of salt treatment, while the content of potassium was significantly lower after 168 h of salt treatment. Root epidermal cells were shed and a more compact layer of cells formed as the treatment duration increased. Salt stress led to deformation of cells and damage to mitochondria in the epidermis and endodermis, whereas stele cells suffered less damage. Transcriptome analysis identified 10,378 differentially expressed genes (DEGs), with more genes showing differential expression after 24 h and 168 h of exposure than after shorter durations of exposure to salinity. Signal transduction and ion transport genes such as HKT and CHX were enriched among DEGs in the early stages (1 h or 6 h) of salt stress, while expression of genes involved in programmed cell death was significantly upregulated at 168 h, corresponding to changes in ion contents and cell structure of roots. Oxidative stress and detoxification genes were also expressed differentially and were enriched among DEGs at different stages. Conclusions. These results not only elucidate the mechanism and the molecular pathway governing salt tolerance, but also serve as a basis for identifying gene function related to salt stress in C. equisetifolia.