文献类型：期刊文献

英文题名：De novo transcriptome assembly of the cotyledon of Camellia oleifera for discovery of genes regulating seed germination

作者：Long, Wei[1] Yao, Xiaohua[1] Wang, Kailiang[1] Sheng, Yu[1] Lv, Leyan[2]

第一作者：龙伟

通信作者：Yao, XH[1]|[a00051e90d3009f1c7c75]姚小华;

机构：[1]Chinese Acad Forestry, Res Inst Subtrop Forestry, Zhejiang Prov Key Lab Tree Breeding, Hangzhou 311400, Zhejiang, Peoples R China;[2]Zhejiang Tongji Vocational Coll Sci & Technol, Dept Hydraul Engn, Hangzhou 311231, Zhejiang, Peoples R China

年份：2022

卷号：22

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000801174900001)】；

基金：This research was funded by National Key R&D Program of China (2019YFD1001602), the Fundamental Research Funds for the Central Public Welfare Scientific Research Institutes (CAFYBB2017SY016).

语种：英文

外文关键词：Camellia oleifera; Cotyledon; Seed germination; Transcriptome; Hormone

摘要：Background Camellia oleifera (C.oleifera) is one of the most important wood oil species in the world. C.oleifera was propagated by nurse seedling grafting. Since the morphology of rootstocks has a significant impact on grafting efficiency and seedling quality, it is necessary to understand the molecular mechanism of morphogenesis for cultivating high-quality and controllable rootstocks. However, the genomic resource for this species is relatively limited, which hinders us from fully understanding the molecular mechanisms of seed germination in C.oleifera. Results In this paper, using transcriptome sequencing, we measured the gene expression in the C.oleifera cotyledon in different stages of development and the global gene expression profiles. Approximately 45.4 gigabases (GB) of paired-end clean reads were assembled into 113,582 unigenes with an average length of 396 bp. Six public protein databases annotate 61.5% (68,217) of unigenes. We identified 11,391 differentially expressed genes (DEGs) throughout different stages of germination. Enrichment analysis revealed that DEGs were mainly involved in hormone signal transduction and starch sucrose metabolism pathways. The gravitropism regulator UNE10, the meristem regulators STM, KNAT1, PLT2, and root-specific transcription factor WOX11 all have higher gene expression levels in the CAM2 stage (seed soaking), which indicates that the cotyledon-regulated program for germination had initiated when the seeds were imbibition. Our data showed differentially reprogrammed to multiple hormone-related genes in cotyledons during C.oleifera seed germination. Conclusion Cotyledons play vital roles, both as the main nutrient provider and as one primary instructor for seed germination and seedling growth. Together, our study will significantly enrich the genomic resources of Camellia and help us understand the molecular mechanisms of the development in the seed germination and seedling growth of C.oleifera. It is helpful to culture standard and superior quality rootstock for C.oleifera breeding.