文献类型：期刊文献

英文题名：The PtobZIP55-PtoMYB170 module regulates the wood anatomical and chemical properties of Populus tomentosa in acclimation to low nitrogen availability

作者：Wu, Jiangting[1] Deng, Shurong[1] Wang, Yang[1] Jia, Chenlin[1] Wei, Jia[1] Zhou, Mengyan[1] Zhu, Dongyue[1] Li, Zhuorong[1] Fayyaz, Payam[2] Luo, Zhi-Bin[1,3,4] Zhou, Jing[1] Shi, Wenguang[1]

第一作者：Wu, Jiangting

通信作者：Luo, ZB[1];Zhou, J[1];Shi, WG[1];Luo, ZB[2];Luo, ZB[3]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[2]Univ Yasuj, Agr & Nat Resources Fac, Forest Range & Watershed Management Dept, Yasuj 7591963179, Iran;[3]Chinese Acad Forestry, Inst Ecol Conservat & Restorat, Beijing 100091, Peoples R China;[4]Comprehens Expt Ctr Chinese Acad Forestry Yellow R, Dongying 257000, Shandong, Peoples R China

年份：2025

卷号：67

期号：1

起止页码：117-134

外文期刊名：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

收录：;Scopus(收录号：2-s2.0-85209198367);WOS:【SCI-EXPANDED(收录号:WOS:001356909900001)】；

基金：This work was supported by the National Key Research and Development Program of China (Grant nos. 2022YFD2201400 and 2016YFD0600105), the National Natural Science Foundation of China (Grant nos. 32371826 and 32371841), and the Key Research and Development Program of Shandong Province (Grant no. 2023CXPT026). The authors would like to thank the Research Core Facility at the State Key Laboratory of Tree Genetics and Breeding, for supporting this work. Professor Keming Luo (Southwest University of China) is highly appreciated for providing the PtoMYB170-pYLCRIPSR/Cas9 vector and the assistance for plant transformation.

语种：英文

外文关键词：lignin biosynthesis; nitrogen deficiency; Populus; secondary xylem; transcriptional regulation; wood formation

摘要：Poplar plantations are often established on nitrogen-poor land, and poplar growth and wood formation are constrained by low nitrogen (LN) availability. However, the molecular mechanisms by which specific genes regulate wood formation in acclimation to LN availability remain unclear. Here, we report a previously unrecognized module, basic region/leucine zipper 55 (PtobZIP55)-PtoMYB170, which regulates the wood formation of Populus tomentosa in acclimation to LN availability. PtobZIP55 was highly expressed in poplar wood and induced by LN. Altered wood anatomical properties and increased lignification were detected in PtobZIP55-overexpressing poplars, whereas the opposite results were detected in PtobZIP55-knockout poplars. Molecular and transgenic analyses revealed that PtobZIP55 directly binds to the promoter sequence of PtoMYB170 to activate its transcription. The phenotypes of PtoMYB170 transgenic poplars were similar to those of PtobZIP55 transgenic poplars under LN conditions. Further molecular analyses revealed that PtoMYB170 directly bound the promoter sequences of lignin biosynthetic genes to activate their transcription to increase lignin concentrations in LN-treated poplar wood. These results suggest that PtobZIP55 activates PtoMYB170 transcription, which in turn positively regulates lignin biosynthetic genes, increasing lignin deposition in the wood of P. tomentosa in the context of acclimation to LN availability.