文献类型：期刊文献

英文题名：Identification of random amplified polymorphic DNA markers linked to sex determination in Calamus simplicifolius C. F. Wei

作者：Yang, H[] Gan, SM[] Yin, GT[] Xu, HC[]

第一作者：杨怀

通信作者：Yin, GT[1]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[2]Zhejiang Acad Forestry, Hangzhou 310023, Peoples R China

年份：2005

卷号：47

期号：10

起止页码：1249-1253

外文期刊名：JOURNAL OF INTEGRATIVE PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000232340700010)】；

语种：英文

外文关键词：Calamus simplicifolius; random amplified polymorphic DNA; rattan; sex determination

摘要：The random amplified polymorphic DNA (RAPD) molecular marker technique was used to determine the sex of Calamus simplicifolius C. F. Wei In the present study, DNA samples were extracted individually from 10 male and 10 female plants. After a total of 1040 decamer primers had been tested, an approximate 500-bp male-specific DNA fragment was generated with the S 1443 primer. It is feasible to identify sex at the early stages of plant life, which is beneficial for improving breeding programs of this dioecious species. In addition, we have obtained a proper RAPD protocol that is useful for other species of rattan.