文献类型：期刊文献

英文题名：The WUSCHELa (PtoWUSa) is Involved in Developmental Plasticity of Adventitious Root in Poplar

作者：Li, Jianbo[1,2] Jia, Huixia[2,3] Sun, Pei[2,3] Zhang, Jin[4] Xia, Yongxiu[1] Hu, Jianjun[2,3] Wang, Lijuan[2,3] Lu, Mengzhu[5]

通信作者：Wang, LJ[1];Wang, LJ[2];Lu, MZ[3]|[a0005e27922ec495458cc]王丽娟;

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, Beijing 102300, Peoples R China;[2]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing 100091, Peoples R China;[3]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[4]Oak Ridge Natl Lab, Biosci Div, Oak Ridge, TN 37831 USA;[5]Zhejiang Agr & Forestry Univ, Coll Forestry & Biotechnol, Hangzhou 311300, Peoples R China

年份：2020

卷号：11

期号：2

外文期刊名：GENES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000519271500051)】；

基金：This work was supported by the National Key Program on Transgenic Research (2018ZX08020002), the National Natural Science Foundation of China (31800569), and Fundamental Research Funds of Chinese Academy of Forestry (CAFYBB2019ZY003).

语种：英文

外文关键词：adventitious roots; auxin; poplar; PtoWUSa; transgenic plant

摘要：WUSCHEL-RELATED HOMEOBOX (WOX) transcription factors play critical roles in cell fate determination during plant development. As the founding member of the WOX family, WUSCHEL (WUS) is characterized for its role in maintaining stem cell in meristem. In this study, we investigated the function of Populus tomentosa WUSCHELa (PtoWUSa) in adventitious roots (ARs) in poplar. Expression profile analysis showed that PtoWUSa was not only expressed in shoot apical meristem and stem, but also expressed in ARs. Ectopic expression of PtoWUSa in Arabidopsis resulted in shortened primary root, as well as agravitropism and multiple branches. Overexpression of PtoWUSa in poplar increased the number of ARs but decreased their length. Moreover, the AR tip and lateral root tip became larger and swollen. In addition, the expression of auxin transporter genes PIN-FORMED were downregulated in ARs of transgenic plant. Taken together, these results suggest that PtoWUSa could be involved in AR development in poplar through regulating the polar auxin transport in ARs.