文献类型：期刊文献

英文题名：Variation in microbial CAZyme families across degradation severity in a steppe grassland in northern China

作者：Zhang, Qian[1] Xu, Xiaoqing[1] Duan, Junguang[1] Koide, Roger T.[2] Xu, Lei[3] Chu, Jianmin[1]

第一作者：张倩

通信作者：Chu, JM[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing, Peoples R China;[2]Brigham Young Univ, Dept Biol, Provo, UT USA;[3]Urumqi Seedling Farm, Urumqi, Xinjiang, Peoples R China

年份：2023

卷号：11

外文期刊名：FRONTIERS IN ENVIRONMENTAL SCIENCE

收录：;Scopus(收录号：2-s2.0-85149637432);WOS:【SCI-EXPANDED(收录号:WOS:000943781700001)】；

基金：Funding This work was supported by the Strategic Priority Research Program of Chinese Academy of Sciences (Grant No. XDA26020102), the Central Public-interest Scientific Institution Basal Research Fund (CAFYBB2020ZB001) and the Natural Science Foundation of China (No. 31870099).

语种：英文

外文关键词：grassland degradation; CAZyme; soil organic carbon; plant community; metagenomics

摘要：Little is known about the effects of grassland degradation on the carbohydrate-active enzyme (CAZYme) genes responsible for C cycling. Here we used a metagenomic approach to reveal variation in abundance and composition of CAZyme genes in grassland experiencing a range of degradation severity (i.e., non-, light, moderately, and severely degraded) in two soil layers (0-10 cm, 10-20 cm) in a steppe grassland in northern China. We observed a higher CAZyme abundance in severely degraded grassland compared with the other three degradation severities. Glycoside hydrolase (GH) and glycosyltransferase (GT) were identified as the most abundant gene families. The Mantel test and variation partitioning suggested an interactive effect of degradation severity and soil depth with respect to CAZyme gene composition. Structural equation modeling indicated that total soil carbon, microbial biomass carbon and organic carbon were the three soil characteristics most important to CAZyme abundance, which suggests an interaction between degradation and soil carbon fractions in determining CAZyme gene composition. Both above- and below-ground factors linked to soil organic matter play a central role in determining the abundance of CAZyme gene families.