文献类型：期刊文献

中文题名：短叶省藤离体快繁研究

英文题名：Tissue Culture of Calamus egregius

作者：曾炳山[1]

第一作者：曾炳山

机构：[1]中国林业科学研究院热带林业研究所

年份：1997

卷号：17

期号：4

起止页码：37-43

中文期刊名：中南林学院学报

外文期刊名：JOURNAL OF CENTRAL SOUTH FORESTRY UNIVERSITH

收录：CSCD:【CSCD_E2011_2012】；

基金：国家"八五"科技攻关项目;国家自然科学基金

语种：中文

中文关键词：藤;短叶省藤;丛芽诱导;继代培养;离体培养

外文关键词：Calamus egregius;tissue culture;subculture;outplanting

分类号：S564.103.5

摘要：以短叶省藤种胚作材料进行组培苗快繁研究，结果表明．种胚成熟度对丛芽诱导有显著的影响，提出了采收种子的各项最佳指标，筛选出了最优培养基及最代继代培养基优化组合，为短叶省藤快繁探索出了新的途径．同时．进行了组培苗移植试验，解决了组培苗田间移植的技术关键，提出了适宜移栽组织苗的各项生长指标，筛选出了最佳组合的移植介质．
Calamus egregius Burret is a vernacular rattan species with high cane quality,and is distributed within Hainan I8land with a broad adaptability. Studies have been made of explant selection, disinfection,de-differentiation, prescription of medium, rooting and outplanting. If embryos are selected as explants, then the proper sampling period is important for a high ratio of clumping buds. After de-differentiation and culture on a modified MS medium supplemented with BA, IBA, 2, 4-D,the embryos' reproductive multiple can reach 10. 03 per month. Induced by NAA, in vitro seedlings are apt to take root. Outplanted with a height>4. 0 cm and a total length of roots>4. 0 cm, the survival rate of seedlings can reach a percentage more than 95%.