文献类型：期刊文献

中文题名：鉴别抗杨叶枯病基因连锁分子标记

英文题名：IDENTIFICATION OF RAPD MOLECULAR MARKERS FOR RESISTANCE AGAINST ALTERNARIA ALTERNATA IN POPLAR

作者：苏晓华[1] 张香华[1] 李金花[1] 张绮纹[1] 郑先武[2]

第一作者：苏晓华

机构：[1]中国林业科学研究院林业研究所;[2]中国科学院遗传研究所

年份：2000

卷号：36

期号：6

起止页码：73-76

中文期刊名：林业科学

外文期刊名：SCIENTIA SILVAE SINICAE

收录：CSTPCD;;北大核心:【北大核心1996】；CSCD:【CSCD2011_2012】；

基金：国家"九五"攻关专项!"杨树纸浆材用材林树种良种选育及培育技术研究"部分研究内容

语种：中文

中文关键词：杨叶枯病;分子标记;抗病基因;杨树;连锁分子标记

外文关键词：Alternaria alternata, Molecular marker, Resistant gene, Poplar

分类号：S763.721.1

摘要：本项研究是以抗杨叶枯病 (Alternariaalternata (Fr.)Keissler)美洲黑杨 (PopulusdeltoidesMarsh .)(♀ )与感病的青杨 (P .cathayanaRehd .) (♂ )及它们的种间杂种F1代和F2 代为材料 ,经室内外人工接种表型抗性鉴定 ,认为美洲黑杨对杨叶枯病的抗性Ala是由 1对隐性纯合基因控制。为进一步识别与Ala抗性基因位点相连锁的标记 ,我们采用RAPD分子标记方法与BSA(BulkedSegregantAnalysis)分群法结合 ,用 4 0 0个 1 0 mer随机引物 ,分析了大约 4 2 0 0条DNA片断 ,鉴别出与Ala位点紧密相连的分子标记 2个 (RPH1 2 6和RPH1 2 4 )。Ala抗病基因位于第 3连锁群 ,2个标记距Ala基因遗传距离均为 3 60cM。
In the present study a three\|generation Populus deltoides (R)× P.cathayana (S) hybrid poplar pedigree,comprising F\-1 and F\-2 progenies,was used to investigate the genetic control of resistance to Alternaria alternata (Ala).The susceptibility of parents,F\-1 and F\-2 to Ala was tested in both the greenhouse and the field.The results suggested that the resistance be determined by a single recessive gene for P.deltoides. To identify the markers linked to Ala resistance,using RAPD markers,in combination with bulked segregant analysis,we analyzed approximately 4200 selectively amplified DNA fragments using 400 primers and identified two markers linked to this resistance gene. Ala is mapped on group 3,and 3.60 cM from RPH12\|6 or RPH12\|4.The results are the basis for molecular marker assisted selection and early identification of disease resistance varieties.