文献类型：期刊文献

中文题名：地涌金莲野生与栽培种群遗传多样性RAPD分析

英文题名：RAPD Analysis on the Genetic Diversity of Wild and Cultivated Populations of Musella lasiocarpa

作者：潘庆杰[1] 李正红[1] 王雁[2] 田杰[1] 谷勇[1] 刘秀贤[1]

第一作者：潘庆杰

机构：[1]中国林业科学研究院资源昆虫研究所;[2]中国林业科学研究院林业研究所

年份：2007

卷号：20

期号：5

起止页码：668-672

中文期刊名：林业科学研究

外文期刊名：Forest Research

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家环保总局项目"中国重点观赏植物种质资源调查"(2004-11-07)的部分内容

语种：中文

中文关键词：地涌金莲;RAPD;遗传多样性;聚类分析

外文关键词：Musella lasiocarpa ; RAPD ; genetic diversity ; cluster analysis

分类号：Q75

摘要：利用RAPD分子标记技术对采自滇川两省的12个地涌金莲野生和栽培种群进行遗传多样性分析。选择10条随机引物在12个种群中共扩增出88条带，其中多态性带85条，整个种的多态性位点百分比PPB为96．59％，遗传多样性指数日为0．2890以及Shannon信息指数，为0．4416。种群间的遗传分化系数Gst为0．5682，即43．18％的遗传变异来自于种群内，56．82％的遗传变异来自于种群间，种群问的遗传分化水平略高于种群内。种群问遗传一致度变化范围为在0．66～0．95之间。聚类结果显示：野生种群之间遗传距离较近，与地理分布基本相一致；栽培种群遗传距离较远，与地理分布不一致。
Random amplified polymorphic DNA （RAPD）was amplified to study the genetic diversity and genetic structure of Musella lasiocarpa based on twelve wild and cultivated populations. 10 primers were screened to use, and 88 bands were amplified, among which 85 were polymorphic. At specific level, the percentage of polymorphic bands, the genetic diversity index and Shannon information index were 96.59%, 0. 289 0 and 0.441 6. The total gene differentiation （Gst） was 0. 568 2, which indicated 43.13% of genetic variation resided within populations and slightly higher than that among populations. The genetic identity among populations ranged from 0.66 to 0.95. The results from cluster analysis showed that the genetic distance among wild populations was close, and was con- sistent with their geographical distribution pattern; the genetic distance among cultivated populations was far and was not consistent with their geographical distribution pattern.