文献类型：期刊文献

英文题名：Rapid and Efficient Detection of 16SrI Group Areca Palm Yellow Leaf Phytoplasma in China by Loop-Mediated Isothermal Amplification

作者：Yu, Shao-shuai[1] Che, Hai-yan[2] Wang, Sheng-jie[3] Lin, Cai-li[4] Lin, Ming-xing[1] Song, Wei-wei[1] Tang, Qing-hua[1] Yan, Wei[1] Qin, Wei-quan[1]

第一作者：Yu, Shao-shuai

通信作者：Qin, WQ[1]

机构：[1]Chinese Acad Trop Agr Sci, Coconut Res Inst, Wenchang 571339, Peoples R China;[2]Chinese Acad Trop Agr Sci, Environm & Plant Protect Inst, Haikou 571101, Hainan, Peoples R China;[3]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[4]Chinese Acad Forestry, Res Inst Forest Ecol Environm & Protect, Beijing 100091, Peoples R China

年份：2020

卷号：36

期号：5

起止页码：459-467

外文期刊名：PLANT PATHOLOGY JOURNAL

收录：;Scopus(收录号：2-s2.0-85091745469);WOS:【SCI-EXPANDED(收录号:WOS:000576366000008)】；

基金：This research was supported by the Hainan Major Research Project for Science and Technology, China (zdkj201817).

语种：英文

外文关键词：Areca palm yellow leaf disease; loop-mediated isothermal amplification; phytoplasma; rapid detection; 16S rRNA gene sequence

摘要：Areca palm yellow leaf (AYL) disease caused by the 16SrI group phytoplasma is a serious threat to the development of the Areca palm industry in China. The 16S rRNA gene sequence was utilized to establish a rapid and efficient detection system efficient for the 16SrI-B subgroup AYL phytoplasma in China by loop-mediated isothermal amplification (LAMP). The results showed that two sets of LAMP detection primers, 16SrDNA-2 and 16SrDNA-3, were efficient for 16SrIB subgroup AYL phytoplasma in China, with positive results appearing under reaction conditions of 64 degrees C for 40 min. The lowest detection limit for the two LAMP detection assays was the same at 200 ag/mu l, namely approximately 53 copies/mu l of the target fragments. Phytoplasma was detected in all AYL disease samples from Baoting, Tunchang, and Wanning counties in Hainan province using the two sets of LAMP primers 16SrD-NA-2 and 16SrDNA-3, whereas no phytoplasma was detected in the negative control. The LAMP method established in this study with comparatively high sensitivity and stability, provides reliable results that could be visually detected, making it suitable for application and research in rapid diagnosis of AYL disease, detection of seedlings with the pathogen and breeding of diseaseresistant Areca palm varieties.