文献类型：期刊文献

英文题名：Construction of a high-density genetic map and QTL mapping of leaf traits and plant growth in an interspecific F-1 population of Catalpa bungei x Catalpa duclouxii Dode

作者：Lu, Nan[1] Zhang, Miaomiao[1] Xiao, Yao[1] Han, Donghua[2] Liu, Ying[3] Zhang, Yu[1] Yi, Fei[1] Zhu, Tianqing[1] Ma, Wenjun[1] Fan, Erqin[1,4] Qu, Guanzheng[4] Wang, Junhui[1]

第一作者：卢楠

通信作者：Wang, JH[1]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Key Lab Tree Breeding & Cultivat State Forestry A, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Nanjing Forestry Univ, Coll Landscape Architecture, Nanjing 210037, Jiangsu, Peoples R China;[3]Northwest A&F Univ, Coll Forestry, Yangling 712100, Shaanxi, Peoples R China;[4]Northeast Forestry Univ, State Key Lab Tree Genet & Breeding, Harbin, Peoples R China

年份：2019

卷号：19

期号：1

外文期刊名：BMC PLANT BIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000508577600003)】；

基金：This work was financially supported by The National Key Research and Development Program of China (2017YFD0600201). The funders had no role in the study design, data analysis and interpretation, and manuscript writing, but just provided the financial.

语种：英文

外文关键词：Catalpa bungei; Genetic map; RAD-seq; QTL mapping; Leaf traits; Plant growth

摘要：Background: Catalpa bungei is an important tree species used for timber in China and widely cultivated for economic and ornamental purposes. A high-density linkage map of C. bungei would be an efficient tool not only for identifying key quantitative trait loci (QTLs) that affect important traits, such as plant growth and leaf traits, but also for other genetic studies. Results: Restriction site-associated DNA sequencing (RAD-seq) was used to identify molecular markers and construct a genetic map. Approximately 280.77 Gb of clean data were obtained after sequencing, and in total, 25, 614,295 single nucleotide polymorphisms (SNPs) and 2,871,647 insertions-deletions (InDels) were initially identified in the genomes of 200 individuals of a C. bungei (7080) x Catalpa duclouxii (16-PJ-3) F-1 population and their parents. Finally, 9072 SNP and 521 InDel markers that satisfied the requirements for constructing a genetic map were obtained. The integrated genetic map contained 9593 pleomorphic markers in 20 linkage groups and spanned 3151.63 cM, with an average distance between adjacent markers of 0.32 cM. Twenty QTLs for seven leaf traits and 13 QTLs for plant height at five successive time points were identified using our genetic map by inclusive composite interval mapping (ICIM). Q16-60 was identified as a QTL for five leaf traits, and three significant QTLs (Q9-1, Q18-66 and Q18-73) associated with plant growth were detected at least twice. Genome annotation suggested that a cyclin gene participates in leaf trait development, while the growth of C. bungei may be influenced by CDC48C and genes associated with phytohormone synthesis. Conclusions: This is the first genetic map constructed in C. bungei and will be a useful tool for further genetic study, molecular marker-assisted breeding and genome assembly.