文献类型：期刊文献

英文题名：Tissue-specific accumulation and subcellular localization of chalcone isomerase (CHI) in grapevine

作者：Wang, Huiling[1,3] Wang, Wei[2] Zhan, Jicheng[4] Huang, Weidong[4] Xu, Haiying[1,5]

第一作者：Wang, Huiling

通信作者：Xu, HY[1];Huang, WD[2];Xu, HY[3]

机构：[1]Beijing Acad Forestry & Pomol Sci, Beijing 100097, Peoples R China;[2]Chinese Acad Forestry, Inst Forestry, Key Lab Silviculture State Forestry Adm, Beijing 100091, Peoples R China;[3]Minist Agr, Key Lab Urban Agr North China, Shanghai, Peoples R China;[4]China Agr Univ, Coll Food Sci & Nutr Engn, Beijing 100083, Peoples R China;[5]Beijing Engn Res Ctr Deciduous Fruit Trees, Beijing 100097, Peoples R China

年份：2019

卷号：137

期号：1

起止页码：125-137

外文期刊名：PLANT CELL TISSUE AND ORGAN CULTURE

收录：;EI(收录号：20190406403584);Scopus(收录号：2-s2.0-85060144558);WOS:【SCI-EXPANDED(收录号:WOS:000462031300012)】；

基金：This study was funded by the special fund of China Agriculture Research System (CARS-29), the National Natural Science Foundation of China (No. 31601712), Beijing Municipal Natural Science Foundation (No. 6182007), and the Natural Science Foundation of Beijing Academy of Agriculture and Forestry Science (No. QNJJ201604).

语种：英文

外文关键词：Grapevine; Chalcone isomerase; Flavonoids; Immunolocalization

摘要：Flavonoids are widely distributed secondary metabolic products with many biological functions in plants. Further elucidation of the accumulation and localization patterns of its biosynthesis enzymes will broaden our understanding of flavonoids biosynthesis and regulation. Chalcone isomerase (CHI, EC 5.5.1.6) is an early-step enzyme in the flavonoids biosynthesis pathway. In this study, using an antibody specifically developed against grapevine CHI enzyme, we found that the accumulation of CHI protein exhibited temporal and spatial specificity. In grape berries, CHI was investigated mainly in the outer hypodermis cells of exocarp tissues, in the vascular bundles of mesocarp; and in the integument and the cells around the raphe of seeds. At the subcellular level, CHI was visualized in the cytoplasm, nucleus, and plastids (chloroplasts) of the exocarp cells, while only in the cytoplasm of mesocarp vascular bundle cells. In grapevine vegetable organs, the leaf mesophyll and phloem of leaf veins, the pith ray and primary phloem of stems, the primary phloem and endoderm of roots, and the young leaves, leaf primordium, and the growth point of leaf buds were CHI signal-positive. In these tissue cells, CHI was primarily observed in the cytoplasm, cell wall, and nucleus. The distinct localization patterns of CHI suggested the complexity of flavonoids biosynthesis in grapevine. Key MessageThe distribution of CHI protein exhibited temporal and spatial specificity. Different subcellular localization patterns were observed in grape berries and grapevine vegetable tissues.