文献类型：期刊文献

英文题名：Overexpression of Anthocyanidin Reductase Increases Flavonoids Content to Combat Fusarium Wilt in the Root Xylem of Vernicia montana

作者：Wang, Jia[1,2,3] Yang, Yang[1,2,3] Gao, Ming[1,2,3] Zhao, Yunxiao[1,2,3] Chen, Yicun[1,2,3] Wang, Yangdong[1,2,3]

第一作者：Wang, Jia;王佳

通信作者：Chen, YC[1];Wang, YD[1];Chen, YC[2];Wang, YD[2];Chen, YC[3];Wang, YD[3]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Zhejiang, Peoples R China;[3]Zhejiang Key Lab Forest Genet & Breeding, Hangzhou, Peoples R China

年份：2026

卷号：27

期号：1

外文期刊名：MOLECULAR PLANT PATHOLOGY

收录：;Scopus(收录号：2-s2.0-105027059369);WOS:【SCI-EXPANDED(收录号:WOS:001659120300001)】；

基金：This work was supported by grants from the National Natural Science Foundation of China (32572095) and the Ten Thousand People Plan of Science and Technology Innovation Leading Talent of Zhejiang, China (2022R52028).

语种：英文

外文关键词：anthocyanidin reductase; flavonoid biosynthesis; Fusarium wilt disease; root xylem; Vernicia montana

摘要：Tung wilt disease, caused by Fusarium oxysporum f. sp. fordiis (Fof-1), poses a serious threat to tung oil tree (Vernicia fordii) production. Fortunately, another native Vernicia species in China, V. montana (woody tung oil tree) exhibits high resistance ability to the pathogen. The resistant and susceptible tung trees provide material for the investigation on the mechanism underlying the resistance to Fusarium wilt disease. Root xylem extracts of resistant V. montana significantly inhibited Fof-1 growth compared with that of susceptible V. fordii. Metabolomic analysis of V. montana root xylem revealed that 13 types of flavonoids increased after Fof-1 infection. Of the 13 flavonoids, antimicrobial assays showed that catechin, (-)-epicatechin and (-)-epigallocatechin exhibited an obvious inhibitory effect on Fof-1 growth. Transcriptomic analysis revealed that several genes with up-regulated expression patterns were also enriched in the flavonoid biosynthesis pathway after Fof-1 infection in V. montana. Among them, the anthocyanidin reductase (ANR) gene is directly involved in the biosynthesis of antimicrobial (-)-epicatechin and (-)-epigallocatechin. Moreover, transgenic V. montana lines overexpressing the VmANR gene elevated eight types of flavonoid concentrations, and silencing VmANR resulted in a substantial reduction in the levels of catechin and myricitrin. The enzyme activity assay in vitro further verified that VmANR catalysed the formation of (-)-epigallocatechin from the substrate cyanidin. This study identifies VmANR as a critical gene to promote biosynthesis of antimicrobial flavonoids in shaping resistance to Fof-1 infection, and offers an effective strategy for breeding Fusarium-resistant tung oil trees.