文献类型：期刊文献

中文题名：用范氏法测定多类植物中酸性洗涤木质素的影响因素研究

英文题名：Research on Factors Affecting Determination of Acid Detergent Lignin in Many Types of Plants by Using Van Soest Method

作者：李朝英[1] 郑路[1,2] 杨文娟[1] 莫世宇[1]

第一作者：李朝英

机构：[1]中国林业科学研究院热带林业实验中心;[2]广西友谊关森林生态系统国家定位观测研究站

年份：2019

卷号：0

期号：3

起止页码：61-64

中文期刊名：江西农业学报

外文期刊名：Acta Agriculturae Jiangxi

收录：CSTPCD

基金：广西自然科学基金项目"马尾松人工林采伐剩余物分解及提升地力研究"(2013GXNSFAA019106)

语种：中文

中文关键词：范氏法;植物;酸性洗涤木质素;测定;影响因素

外文关键词：Van Soest method;Plant;ADL;Determination;Influencing factor

分类号：Q946

摘要：探讨了用范氏法测定植物中酸性洗涤木质素(ADL)的影响因素,对不同消煮时间、不同硫酸浓度、不同浸泡时间条件下的测定结果进行了比较分析。结果表明:样品消煮的适宜时间为30 min,硫酸适宜浓度为72%～75%,浸泡适宜时间为3～4 h;人工检测结果的变异系数(CV)在2.23%～6.66%之间,仪器法的CV在0.15%～1.10%之间;人工检测与仪器法所测结果无显著性差异;范氏法与王玉万法所测结果无显著性差异,这两种方法适用于植物ADL的测定。
The author explored the factors affecting the determination of plant acid detergent lignin(ADL) by using Van Soest method, and compared and analyzed the determination results under different conditions. The results showed that: the suitable time for sample boiling was 30 min;the suitable concentration of sulfuric acid for sample decomposition was 72%~75%;the appropriate time for sample soaking was 3~4 h;the coefficient of variation(CV) of manual determination results was 2.23%~6.66%, while the CV of instrument determination results was 0.15%~1.10%;there was no significant difference in determination results between manual method and instrument method;there was also no significant difference in determination results between Van Soest method and Wang Yuwan’s method, and these two methods were all suitable for the determination of plant ADL.