文献类型：期刊文献

英文题名：Development of SSR Markers in Hickory (Carya cathayensis Sarg.) and Their Transferability to Other Species of Carya

作者：Li, Juan[1] Zeng, Yanru[1] Shen, Dengfeng[2] Xia, Guohua[1] Huang, Yinzhi[1] Huang, Youjun[1] Chang, Jun[3] Huang, Jianqin[1] Wang, Zhengjia[1]

第一作者：Li, Juan

通信作者：Zeng, YR[1]

机构：[1]Zhejiang A&F Univ, Nurturing Stn State Key Lab Subtrop Silviculture, Linan 311300, Zhejiang, Peoples R China;[2]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing 100083, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Fuyang 311400, Peoples R China

年份：2014

卷号：15

期号：5

起止页码：357-379

外文期刊名：CURRENT GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000345225600005)】；

基金：This work is supported by grants "Linkage analysis-based genetic study of apomitic hickory (Carya cathayensis Sarg.) (31370678)" and "Mechanism of apomixis in Carya catyayensis and its biological significance in breeding system (31100229)" from NSFC (Natural Science Foundation of China), a grant (2011R50030) from the Program for Zhejiang Leading Team of S& T Innovation, grants (LZ13C160002 and Y3110311) from the NSF of Zhejiang Province, China and a grant (2013AA102605) from "863" Program of China.

语种：英文

外文关键词：Carya cathayensis Sarg.; SSR (Simple sequence repeat); C. hunanensis; C. illinoensis (pecan); C. dabieshanensis

摘要：Hickory (Carya cathayensis Sarg.), an important nut-producing species in Southeastern China, has high economic value, but so far there has been no cultivar bred under species although it is mostly propagated by seeding and some elite individuals have been found. It has been found recently that this species has a certain rate of apomixis and poor knowledge of its genetic background has influenced development of a feasible breeding strategy. Here in this paper we first release SSR (Simple sequence repeat) markers developed in this species and their transferability to other three species of the same genus, Carya. A total of 311 pairs of SSR primers in hickory were developed based on sequenced cDNAs of a fruit development-associated cDNA library and RNA-seq data of developing female floral buds and could be used to distinguish hickory, C. hunanensis Cheng et R. H. Chang ex R. H. Chang et Lu, C. illinoensis K. Koch (pecan) and C. dabieshanensis M. C. Liu et Z. J. Li, but they were monomorphic in both hickory and C. hunanensis although multi-alleles have been identified in all the four species. There is a transferability rate of 63.02% observed between hickory and pecan and the markers can be applied to study genetic diversity of accessions in pecan. When used in C. dabieshanensis, it was revealed that C. dabieshanensis had the number of alleles per locus ranging from 2 to 4, observed heterozygosity from 0 to 0.6667 and expected heterozygosity from 0.333 to 0.8667, respectively, which supports the existence of C. dabieshanensis as a separate species different from hickory and indicates that there is potential for selection and breeding in this species.