文献类型：期刊文献

英文题名：Comprehensive identification of bHLH transcription factors in Litsea cubeba reveals candidate gene involved in the monoterpene biosynthesis pathway

作者：Yang, Jiahui[1,2] Chen, Yicun[1,2] Gao, Ming[1,2] Wu, Liwen[1,2] Xiong, Shifa[1,2] Wang, Siqi[1,2] Gao, Jing[1,2] Zhao, Yunxiao[1,2] Wang, Yangdong[1,2]

通信作者：Zhao, YX[1];Wang, YD[1];Zhao, YX[2];Wang, YD[2]

机构：[1]Chinese Acad Forestry, State Key Lab Tree Genet & Breeding, Beijing, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou, Zhejiang, Peoples R China

年份：2022

卷号：13

外文期刊名：FRONTIERS IN PLANT SCIENCE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000906981400001)】；

基金：This work was supported by the National Natural Science Foundation of China (32101561, 32071804); Zhejiang Science and Technology Major Program on Agricultural New Variety Breeding [2021C02070-3].; Fundamental Research Funds of CAF [Grant No. CAFYBB2020QA002]; The National Science and Technology Basic Resources Survey Program of China [2019FY100803_05].

语种：英文

外文关键词：bHLH gene family; plant secondary metabolism; Litsea cubeba; genome-wide identification; terpenoids

摘要：Litsea cubeba (Lour.) Person, an economically important aromatic plant producing essential oils, has lemon-like fragrance and 96.44-98.44% monoterpene contents. bHLH transcription factor plays an important role in plant secondary metabolism and terpene biosynthesis. In this study, we used bioinformatics to identify bHLH transcription factors in L. cubeba, 173 bHLH genes were identified from L. cubeba and divided these into 26 subfamilies based on phylogenetic analysis. The majority of bHLHs in each subfamily shared comparable structures and motifs. While LcbHLHs were unevenly distributed across 12 chromosomes, 10 tandem repeats were discovered. Expression profiles of bHLH genes in different tissues demonstrated that LcbHLH78 is a potential candidate gene for regulating monoterpene biosynthesis. LcbHLH78 and the terpene synthase LcTPS42 showed comparable expression patterns in various tissues and fruit development stages of L. cubeba. Subcellular localization analysis revealed that LcbHLH78 protein localizes to the nucleus, consistent with a transcription factor function. Importantly, transient overexpression of LcbHLH78 increased geraniol and linalol contents. Our research demonstrates that LcbHLH78 enhances terpenoid biosynthesis. This finding will be beneficial for improving the quality of L. cubeba and provides helpful insights for further research into the control mechanism of LcbHLH genes over terpenoid biosynthesis.