文献类型：期刊文献

中文题名：巨桉无性系Eg5的卡那霉素和头孢霉素敏感性研究

英文题名：Study on kanamycin and cefotaxime sensitivity of Eucalyptus grandis clone Eg5

作者：郭利军[1] 曾炳山[1] 刘英[1] 李湘阳[1] 裘珍飞[1]

第一作者：郭利军

机构：[1]中国林业科学研究院热带林业研究所

年份：2012

卷号：32

期号：3

起止页码：75-80

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：CSTPCD;;北大核心:【北大核心2011】；CSCD:【CSCD_E2011_2012】；

基金：863高新技术项目(2011AA100202)

语种：中文

中文关键词：桉树;Eg5;抗生素;根癌农杆菌;敏感性

外文关键词：Eucalyptus; Eg5; antibiotic; Agrobacterium tumefaciens; sensitivity

分类号：S792.39;Q943.2

摘要：以巨桉无性系Eg5叶片为外植体材料,试验了硫酸卡那霉素(kanamycin sulfate,Km)对外植体愈伤组织诱导增殖、芽分化、芽生根的影响,并且试验了头孢霉素(Cefotaxime,Cef)对外植体再生的影响及其对根癌农杆菌GV3101的抑制效果。结果表明:抑制愈伤组织增殖培养的卡那霉素浓度为17.5 mg/L,完全抑制芽分化的卡那霉素浓度为60 mg/L,完全抑制生根的卡那霉素浓度为400 mg/L;Cef在0～500mg/L范围内,0～300mg/L Cef对外植体的生长分化影响最小;共培养结束后的外植体经过500 mg/L的Cef处理后,共培养基中添加100 mg/L的Cef便可以完全抑制农杆菌GV3101的生长。
By taking Eucalyptus grandis colne Eg5 leaves as explants,the effects of Kanamycin sulfate on the callus proliferation,bud differentiation and shoot rooting of leaf explants of Eucalyptus grandis clone Eg5 and the effect of Cefotaxime on explant＇s regeneration and the growth of Agrobacterium tumefaciens GV3101 were studied.The results showed that the concentration of kanamycin for restraining callus proliferation was 17.5 mg/L,that for fully suppressing bud differentiation was 60 mg/L and that for inhibiting shoots rooting was 400 mg/L.Cefotaxime at a dosage from 0 mg/L to 300 mg/L had minimal impact on the differentiation of leaf explants.With a soak of the explants in the solution of 500 mg/L Cefotaxime,the growth of agrobacterium can be fully inhibited at a Cefotaxime dosage of 100 mg/L in medium.