文献类型：期刊文献

英文题名：An Assessment of the Environmental Impacts of Transgenic Triploid Populus tomentosa in Field Condition

作者：Guo, Qi[1] Lu, Nan[1,2] Luo, Zijing[1] Sun, Yuhan[1] Jin, Shaowu[3] Wang, Shaoming[3] Guo, Zhimin[3] Li, Feifei[4] Chen, Shouyi[5] Zhang, Wanke[5] Ji, Qingju[6] Li, Yun[1]

第一作者：Guo, Qi

通信作者：Li, Y[1]

机构：[1]Beijing Forestry Univ, Key Lab Genet & Breeding Forest Trees & Ornamenta, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Natl Engn Lab Tree Breeding,Minist Educ,Coll Biol, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Key Lab Tree Breeding & Cultivat, State Key Lab Forest Genet & Tree Breeding, Res Inst Forestry,State Forestry Adm, Beijing 100091, Peoples R China;[3]Luoning Cty Lv Village State Owned Forest Farm, Luoyang 471700, Henan, Peoples R China;[4]Dongsong Township Govt Luoning Cty, Luoyang 471700, Henan, Peoples R China;[5]Chinese Acad Sci, Inst Genet & Dev Biol, Beijing 100101, Peoples R China;[6]Cangzhou Municipal Forestry Seeding & Cutting Man, Cangzhou 061001, Hebei, Peoples R China

年份：2018

卷号：9

期号：8

外文期刊名：FORESTS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000443254400041)】；

基金：This research was funding by the Science and Technology Development Center of the State Forestry Administration Project (JC-2017-02), the National Science and Technology Major Project of the Ministry of Science and Technology of China (2018ZX08021001-003-002), the National High Technology Research and Development Program of China (2013AA102703), the Science and Technology Development Center of the State Forestry Administration Project (JJ-2012-04), the Fundamental Research Funds for the Central Universities (2016BLPX13), and International Science & Technology Cooperation Program of China (2014DFA31140).

语种：英文

外文关键词：Populus; field trial; environmental risk assessment (ERA); dehydration responsive element (DRE) binding protein

摘要：Populus tomentosa grow rapidly, but are salt susceptible. To quickly and efficiently gain new poplar breeds with better salt resistance, a DREB transcription factor derived from Atriplex hortensis was transformed into triploid Populus tomentosa by our lab, which significantly improved the salt tolerance of host plants. However, environmental impacts of transgenic plants must be assessed before large-scale cultivation in China. Here, we conducted a field trial of AhDREB1 transgenic and non-transgenic triploid Populus tomentosa to assess the impact of transgenic trees on rhizospheric soil microbial communities and allelopathic activity of leaves. No significant differences in the number of soil microbes present were detected between the transgenic lines and the non-transgenic controls. The allelopathic activity of leaves from both the transgenic and non-transgenic lines varied with sampling time, but did not differ significantly between the transgenic and non-transgenic lines. These results indicate that the impact on the environment of AhDREB1 transgenic P. tomentosa did not differ significantly from that of the non-transformed controls for the variables observed in this field trial. We also investigated the persistence of AhDREB1 genes in decomposing transgenic poplar leaf on the soil under natural conditions for five months, and our data indicated that fragments of the genetically modified DNA were not detectable in the field after more than two months. We used a triphenyl tetrazolium chloride test (TTC) (or pollen germination method) and hybridization to test the pollen viability and fertility, respectively, of the transgenic and non-transgenic trees and the results showed that the pollen viability of both the transgenic and non-transgenic trees was extremely low in 2016; the receptor plant may have been sterile.