文献类型：期刊文献

中文题名：油茶乙酰CoA酰基转移酶基因cDNA克隆及序列特征分析

英文题名：Cloning and sequence characterization of cDNA encoding acetyl-CoA C-acetyltransferase in Camellia oleifera

作者：张琳[1] 谭晓风[1] 胡姣[1] 姚小华[2] 林萍[2]

第一作者：张琳

机构：[1]中南林业科技大学经济林育种与栽培国家重点实验室;[2]中国林业科学研究院亚热带林业研究所

年份：2011

卷号：31

期号：8

起止页码：108-112

中文期刊名：中南林业科技大学学报

外文期刊名：Journal of Central South University of Forestry & Technology

收录：CSTPCD;;北大核心:【北大核心2008】；CSCD:【CSCD_E2011_2012】；

基金：国家"十一五"油茶科技支撑计划(2009BADB1B01;2009BADB1B02);湖南省自然科学基金项目(10JJ4022);中南林业科技大学青年基金项目(2009004A)

语种：中文

中文关键词：油茶;乙酰CoA酰基转移酶;快速扩增cDNA末端;生物信息学分析

外文关键词：Camellia oleifera;aeetyl-CoA C-acetyltransferase gene; rapid amplification of cDNA ends （RACE）;bioinformatics analysis

分类号：S794.4

摘要：在已知油茶乙酰COA酰基转移酶基因EST序列基础上,设计6条特异引物,以油茶优良无性系‘湘林1号’种仁总RNA为模板,通过反应体系和反应条件优化,最终扩增出一条700 bp左右的目的片段,将该片段回收、克隆、测序,获得718 bp的cDNA序列,将该序列与油茶乙酰COA酰基转移酶基因进行序列拼接,确定了油茶乙酰COA酰基转移酶基因的全长cDNA序列,将该序列提交至GeneBank,登录号为GU594059。油茶乙酰COA酰基转移酶基因全长cDNA序列为1 495 bp,含有一个1 227 bp的ORF,编码408个氨基酸残基。在氨基酸序列水平上,该基因与胡黄连(P.kurrooa)的乙酰COA酰基转移酶基因的相似性最高,为86%,与稻瘟病菌(M.grisea)的最低,为65%。通过在线预测,油茶乙酰COA酰基转移酶基因编码蛋白的等电点pI为6.19,分子量Mw为41 628.6 Da。本研究为揭示油茶油脂合成规律和油茶的分子育种提供了理论依据和科学基础。
Based on sequence information of acetyl-CoA C-acetyltransferase gene, six pairs of primers in conserved regions of this gene were designed. Total RNA of Xianglin No. 1 seeds was used as template, one fragment of 718 bp was successfully amplified by 5＇RACE strategy. Then the full eDNA of acetyl-CoA C-aeetyltransferase gene was determined by assemblying the 5＇ sequence and known EST sequence that had been registered under GenBank （No. GU594059）. The acetyl-CoA C-aeetyltransferase gene of Camellia oleifera was 1 495 bp long, including a completed ORE of 1 227 bp, encoding 408 amino acids. On the amino acid sequence level, this gene showed the highest similarity （86G） with acetyl-CoA C-acetyltransferase gene of P. kurrooa, and the lowest （65%） with M. grisea. In addition, the predicted pI and Mw of Camellia oleifera acetyl-CoA C-acetyltransferase gene was 6. 19, 41 628. 6 Da, respectively.