文献类型：期刊文献

英文题名：Comprehensive Analyses of Four PtoNF-YC Genes from Populus tomentosa and Impacts on Flowering Timing

作者：Li, Juan[1,2] Gao, Kai[1,3] Yang, Xiaoyu[1] Guo, Bin[1,4] Xue, Yinxuan[1] Miao, Deyu[1] Huang, Sai[1] An, Xinmin[1]

第一作者：Li, Juan;李娟

通信作者：An, XM[1]

机构：[1]Beijing Forestry Univ, Coll Biol Sci & Technol, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Natl Engn Res Ctr Tree Breeding & Ecol Restorat, Beijing 100083, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Key Lab State Forestry & Grassland Adm Trop Fores, Guangzhou 510520, Peoples R China;[3]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Peoples R China;[4]Shanxi Acad Forestry & Grassland Sci, Taiyuan 030012, Peoples R China

年份：2022

卷号：23

期号：6

外文期刊名：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000775297100001)】；

基金：This research was funded by the China National Key R&D Program during the 14th Fiveyear Plan Period "Forest Tree Genome Editing Technology" (2021YFD2200101), and the National Natural Science Foundation of China (No. 31870652 and No.31570661).

语种：英文

外文关键词：flowering time; genome-wide analysis; NF-YC; Populus tomentosa; transgenic plant

摘要：Flowering is an important link in the life process of angiosperms, and it is also an important sign of the transformation of plants from vegetative to reproductive growth. Although the flowering regulation network of Arabidopsis is well-understood, there has been little research on the molecular mechanisms of perennial woody plant flower development regulation. Populus tomentosa is a unique Chinese poplar species with fast growth, strong ecological adaptability, and a long lifecycle. However, it has a long juvenile phase, which seriously affects its breeding process. Nuclear factor-Y (NF-Y) is an important type of transcription factor involved in the regulation of plant flowering. However, there are few reports on PtoNF-Y gene flowering regulation, and the members of the PtNF-YC subfamily are unknown. In this study, four key genes were cloned and analyzed for sequence characteristics, gene structure, genetic evolution, expression patterns, and subcellular localization. The plant expression vector was further constructed, and transgenic Arabidopsis and P. tomentosa plants were obtained through genetic transformation and a series of molecular tests. The flowering time and other growth characteristics were analyzed. Finally, the expression level of flowering genes was detected by quantitative PCR, the interaction between PtoNF-YC and PtoCOL proteins was measured using the yeast two-hybrid system to further explain the flowering regulation mechanism, and the molecular mechanisms by which PtNF-YC6 and PtNF-YC8 regulate poplar flowering were discussed. These results lay the foundation for elucidating the molecular regulation mechanism of PtoNF-YC in flowering and furthering the molecular design and breeding of poplar, while providing a reference for other flowering woody plants.