文献类型：期刊文献

英文题名：Mesorhizobium ephedrae sp nov isolated from the roots of Ephedra przewalskii in Kumtag desert

作者：Liu, Lei[1] Liang, Li-xiong[1] Zhang, Xiao-xia[2] Li, Lu-bin[1] Sun, Qi-wu[1]

第一作者：刘蕾

通信作者：Sun, QW[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, State Forestry Adm, State Key Lab Tree Genet & Breeding,Key Lab Tree, Beijing 100091, Peoples R China;[2]Chinese Acad Agr Sci, Inst Agr Resources & Reg Planning, Key Lab Microbial Resources Collect & Preservat, Minist Agr, Beijing 100081, Peoples R China

年份：2018

卷号：68

期号：11

起止页码：3615-3620

外文期刊名：INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000448920600036)】；

基金：This work was supported by The National Key Technologies R&D Program of China (number 2016YFC050080104).

语种：英文

外文关键词：Mesorhizobium; Mesorhizobium ephedrae; Ephedra przewalskii; endophytic bacterium; Kumtag desert

摘要：Strain 6GN-30(T), a Gram-stain-negative, aerobic, non-spore-forming, rod-shaped, motile bacterium was isolated from Ephedra sinica roots in the Kumtag Desert. On the basis of the 16S rRNA gene sequence, the isolate represented a member of the genus Mesorhizobium of the family Phyllobacteriaceae. The results of a phylogenetic analysis indicated that 6GN-30(T) was phylogenetically related to Mesorhizobium soli NHI-8(T). Strain 6GN-30 T grew at a salinity of 0-1.0% (w/v) NaCl (with optimum growth in the absence of NaCl), pH 6.0-9.0 (optimum 7.0-8.0) and 15-45 degrees C. The major cellular fatty acids were summed feature 8 (C-18:1 omega 7c/C-18:1 omega 6c), C-19:0cyclo omega 8c, iso-C-17:0, C-18:0, and C-16 :0. The draft genome of 6GN-30(T) was 6.11 Mb long, with a DNA G+C content of 66.4 mol%. The average nucleotide identity to M. soli NHI-8(T) was 84.32 %. The strain contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, aminophospholipids and phospholipids. The chemotaxonomic, phylogenetic and phenotypic data indicate that 6GN-30(T) represents a novel species of the genus Mesorhizobium for which the name Mesorhizobium ephedrae sp. nov. is proposed. The type strain is 6GN-30(T) (= ACCC 60073(T) = KCTC 62410(T)).