文献类型：期刊文献

英文题名：Sequencing the genome of Marssonina brunnea reveals fungus-poplar co-evolution

作者：Zhu, Sheng[2] Cao, You-Zhi[2] Jiang, Cong[2] Tan, Bi-Yue[2] Wang, Zhong[3] Feng, Sisi[3] Zhang, Liang[4] Su, Xiao-Hua[1] Brejova, Brona[5] Vinar, Tomas[5] Xu, Meng[2] Wang, Ming-Xiu[2] Zhang, Shou-Gong[1] Huang, Min-Ren[2] Wu, Rongling[2,3] Zhou, Yan[4,6]

第一作者：Zhu, Sheng

通信作者：Zhang, SG[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing, Peoples R China;[2]Nanjing Forestry Univ, Jiangsu Key Lab Poplar Germplasm Enhancement & Va, Nanjing, Jiangsu, Peoples R China;[3]Beijing Forestry Univ, Ctr Computat Biol, Beijing, Peoples R China;[4]Chinese Natl Human Genome Ctr Shanghai, Shanghai MOST Key Lab Hlth & Dis Genom, Shanghai, Peoples R China;[5]Comenius Univ, Fac Math Phys & Informat, Bratislava, Slovakia;[6]Fudan Univ, Sch Life Sci, Dept Microbiol & Microbial Engn, Shanghai 200433, Peoples R China

年份：2012

卷号：13

期号：1

外文期刊名：BMC GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000310337500001)】；

基金：We acknowledge the genomic data provided by JGI (Joint Genome Institute, http://www.jgi.doe.gov/), including the data of Populus trichocarp (v1.1, http://genome.jgi-psf. org/poplar/poplar.home.html) and Melampsora laricispopulina (the stain "98AG31", v1.0, http://genome.jgi.doe.gov/Mellp1/Mellp1.home.html). We also acknowledge the genomic data and analysis tools provided by the Broad Institute of Harvard and MIT (http://www.broadinstitute.org) for Bortytis cinerea (the strain "B05.10", http://www.broadinstitute.org/annotation/genome/botrytis_cinerea), Sclerotinia sclerotiorum (the strain "1980", http://www.broadinstitute.org/annotation/genome/sclerotinia_sclerotiorum/MultiHome.html), Magnaporthe grisea (the strain "70-15", http://www.broadinstitute.org/annotation/genome/magnaporthe_grisea/Genomes-Index.html), Fusarium graminearum (the strain "PH-1", http://www.broadinstitute.org/annotation/genome/fusarium_group/MultiHome.html), Schizosaccharomyces pombe (the stain "972 h-", http://www.broadinstitute.org/annotation/genome/schizosaccharomyces_group/Downloads.html), and Saccharomyces cerevisiae (the stain "RM11-1a", http://www.broadinstitute.org/annotation/genome/saccharomyces_cerevisiae.), and Argo Genome Browser (v1.0.31, http://www.broadinstitute.org/annotation/argo/). We thank Lei Zhang at the Institute of Plant Physiology and Ecology, Chinese Academy of Science, China, for implementing the scaffolding algorithm, and the Shanghai Supercomputer Center of China for computing technical support and services. This work was supported by grants from National Basic Research Program of China (2009CB19100), The Doctorate Fellowship Foundation of Nanjing Forestry University, The Science and Technology Commission of Shanghai Municipality (10QA1400600), The Graduate Research Innovation Project of Jiangsu Province, The Priority Academic Program Development of Jiangsu Higher Education Institutions, The Changjiang Scholars Award and "One-thousand Person Plan" Award.

语种：英文

外文关键词：Marssonina leaf spot; Genome sequencing; Host-pathogen interaction; Poplar

摘要：Background: The fungus Marssonina brunnea is a causal pathogen of Marssonina leaf spot that devastates poplar plantations by defoliating susceptible trees before normal fall leaf drop. Results: We sequence the genome of M. brunnea with a size of 52 Mb assembled into 89 scaffolds, representing the first sequenced Dermateaceae genome. By inoculating this fungus onto a poplar hybrid clone, we investigate how M. brunnea interacts and co-evolves with its host to colonize poplar leaves. While a handful of virulence genes in M. brunnea, mostly from the LysM family, are detected to up-regulate during infection, the poplar down-regulates its resistance genes, such as nucleotide binding site domains and leucine rich repeats, in response to infection. From 10,027 predicted proteins of M. brunnea in a comparison with those from poplar, we identify four poplar transferases that stimulate the host to resist M. brunnea. These transferas-encoding genes may have driven the co-evolution of M. brunnea and Populus during the process of infection and anti-infection. Conclusions: Our results from the draft sequence of the M. brunnea genome provide evidence for genome-genome interactions that play an important role in poplar-pathogen co-evolution. This knowledge could help to design effective strategies for controlling Marssonina leaf spot in poplar.