文献类型：期刊文献

英文题名：Genetic Diversity and Evolution of Bradyrhizobium Populations Nodulating Erythrophleum fordii, an Evergreen Tree Indigenous to the Southern Subtropical Region of China

作者：Yao, Yao[1,2] Wang, Rui[1,2] Lu, Jun Kun[3] Sui, Xin Hua[1,2] Wang, En Tao[1,2,4] Chen, Wen Xin[1,2]

第一作者：Yao, Yao

通信作者：Sui, XH[1]

机构：[1]China Agr Univ, Coll Biol Sci, State Key Lab Agrobiotechnol, Beijing 100094, Peoples R China;[2]China Agr Univ, Coll Biol Sci, Minist Agr, Key Lab Soil Microbiol, Beijing 100094, Peoples R China;[3]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou, Guangdong, Peoples R China;[4]Inst Politecn Nacl, Escuela Nacl Ciencias Biol, Mexico City, DF, Mexico

年份：2014

卷号：80

期号：19

起止页码：6184-6194

外文期刊名：APPLIED AND ENVIRONMENTAL MICROBIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000341887100033)】；

基金：This work was supported by the National Nature Science Foundation of China (project number 31170003) and by the 863 Project (2013AA102802-04) (X.H.S.).

语种：英文

摘要：The nodulation of Erythrophleum fordii has been recorded recently, but its microsymbionts have never been studied. To investigate the diversity and biogeography of rhizobia associated with this leguminous evergreen tree, root nodules were collected from the southern subtropical region of China. A total of 166 bacterial isolates were obtained from the nodules and characterized. In a PCR-based restriction fragment length polymorphism (RFLP) analysis of ribosomal intergenic sequences, the isolates were classified into 22 types within the genus Bradyrhizobium. Sequence analysis of 16S rRNA, ribosomal intergenic spacer (IGS), and the housekeeping genes recA and glnII classified the isolates into four groups: the Bradyrhizobium elkanii and Bradyrhizobium pachyrhizi groups, comprising the dominant symbionts, Bradyrhizobium yuanmingense, and an unclassified group comprising the minor symbionts. The nodC and nifH phylogenetic trees defined five or six lineages among the isolates, which was largely consistent with the definition of genomic species. The phylogenetic results and evolutionary analysis demonstrated that mutation and vertical transmission of genes were the principal processes for the divergent evolution of Bradyrhizobium species associated with E. fordii, while lateral transfer and recombination of housekeeping and symbiotic genes were rare. The distribution of the dominant rhizobial populations was affected by soil pH and effective phosphorus. This is the first report to characterize E. fordii rhizobia.