文献类型：期刊文献

英文题名：Overexpression of AtruLEA1 from Acer truncatum Bunge Enhanced Arabidopsis Drought and Salt Tolerance by Improving ROS-Scavenging Capability

作者：Li, Shaofeng[1] Meng, Huijing[2] Yang, Yanfei[3] Zhao, Jinna[3] Xia, Yongxiu[1] Wang, Shaoli[1] Wang, Fei[1] Zheng, Guangshun[1] Li, Jianbo[1]

第一作者：李少锋

通信作者：Zheng, GS[1];Li, JB[1]

机构：[1]Chinese Acad Forestry, Expt Ctr Forestry North China, State Key Lab Tree Genet & Breeding, Natl Permanent Sci Res Base Warm Temperate Zone Fo, Beijing 100091, Peoples R China;[2]Henan Agr Univ, Coll Forestry, Zhengzhou 450046, Peoples R China;[3]Shanxi Agr Univ, Coll Forestry, Taigu 030801, Peoples R China

年份：2025

卷号：14

期号：1

外文期刊名：PLANTS-BASEL

收录：;Scopus(收录号：2-s2.0-85214465756);WOS:【SCI-EXPANDED(收录号:WOS:001393422500001)】；

基金：This research was funded by the Chinese Academy of Forestry-Special funds for basic scientific research service expenses of the central level public welfare research institutes [CAFYBB2020QD001].

语种：英文

外文关键词：Acer truncatum Bunge; LEA1; drought stress; salt stress; ABA

摘要：Late embryonic developmental abundant (LEA) genes play a crucial role in the response to abiotic stress and are important target genes for research on plant stress tolerance mechanisms. Acer truncatum Bunge is a promising candidate tree species for investigating the tolerance mechanism of woody plants against abiotic stress. In our previous study, AtruLEA1 was identified as being associated with seed drought tolerance. In this study, LEA1 was cloned from A. truncatum Bunge and functionally characterized. AtruLEA1 encodes an LEA protein and is located in the nucleus. Phylogenetic tree analysis revealed a recent affinity of the AtruLEA1 protein to AT3G15760.1. Overexpression of AtruLEA1 resulted in enhanced tolerance of Arabidopsis thaliana to drought and salt stress and heightened the ABA sensitivity. Compared to wild-type (WT) plants, plants with overexpressed AtruLEA1 exhibited increased activities of antioxidant enzymes under drought stress. Meanwhile, the ROS level of transgenic Arabidopsis was significantly less than that of the WT. Additionally, the stoma density and stoma openness of AtruLEA1 Arabidopsis were higher compared to those in the WT Arabidopsis under salt and drought stress conditions, which ensures that the biomass and relative water content of transgenic Arabidopsis are significantly better than those of the WT. These results indicated that AtruLEA1 was involved in salt and drought stress tolerances by maintaining ROS homeostasis, and its expression was positively regulated by abiotic stress. These results indicate a positive role of AtruLEA1 in drought and salt stress and provide theoretical evidence in the direction of cultivating resistant plants.