文献类型：期刊文献

英文题名：Effects and Benefits of Orchid Mycorrhizal Symbionts on Dendrobium officinale

作者：Zhang, Yifan[1] Huo, Wenwen[1] Hou, Jiayi[1] Liu, Lei[2] Yu, Xiaoying[1] Xu, Lu[1]

第一作者：Zhang, Yifan

通信作者：Xu, L[1]

机构：[1]Hunan Agr Univ, Coll Hort, Hunan Midsubtrop Qual Plant Breeding & Utilizat E, Changsha 410128, Peoples R China;[2]Chinese Acad Forestry, Res Inst Forestry, State Key Lab Forest Genet & Tree Breeding, Key Lab Silviculture State Forestry Adm, Beijing 100091, Peoples R China

年份：2022

卷号：8

期号：10

外文期刊名：HORTICULTURAE

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000873083900001)】；

基金：This work was funded by the Postgraduate Scientific Research Innovation Project of Province, grant number No. QL20210170, and the project was supported by Scientific Research Fund of Hunan Provincial Education Department, grant number No. 18B101 and the National Natural Science Foundation of China, grant number No. 31800522.

语种：英文

外文关键词：Dendrobium officinale; orchid mycorrhizal fungi; symbiosis; growth morphology; resistance; functional components

摘要：Dendrobium officinale Kimura et Migo, a highly valued Chinese herbal medicine, is on the verge of extinction in the wild, and is not cultivated efficiently. In this study, we explored the possibility that orchid mycorrhizal fungi (OMF) might improve the growth and cultivation of D. officinale. Serendipita sp., Tulasnella calospora and Tulasnella asymmetrica isolated from three different orchids were co-cultured with sterile seedlings of D. officinale. The seedlings were found to stably coexist with fungi after 60 days of co-culture. The co-culture of T. calospora with plants upregulated the activity of antioxidant enzymes, stimulated the production of osmoregulatory substances and reduced electrical conductivity. Plants with T. calospora had longer roots (141.2%), thicker leaves (58.3%), increased root number (71.4%) and leaf number (11.1%), and increased weight (155.2%) and photosynthetic pigment content (99.6%), relative to controls. The content of total medicinal polysaccharides increased by 42.69 % due to the addition of T. calospora. T. asymmetrica was less effective, followed by Serendipita sp. When T. calospora established a symbiotic relationship with D. officinale, resistance indicators increased. The content of functional components was significantly increased. This study contributes to the protection and commercial reproduction of endangered orchid plants with mycorrhizal technology.