文献类型：期刊文献

中文题名：白皮松保育遗传学——天然群体遗传多样性评价与保护策略

英文题名：Conservation Genetics of Pinus bungeana ——Evaluation and Conservation of Natural Populations' Genetic Diversity

作者：李斌[1] 顾万春[1]

第一作者：李斌

机构：[1]中国林业科学研究院林业研究所

年份：2005

卷号：41

期号：1

起止页码：57-64

中文期刊名：林业科学

外文期刊名：Scientia Silvae Sinicae

收录：CSTPCD;;Scopus;北大核心:【北大核心2004】；CSCD:【CSCD2011_2012】；

基金：国家十五攻关项目"林木种质保存技术创新与利用研究"(2 0 0 1-13 );国家基础性工作项目"林木种质资源收集保存"(2 0 0 2 ;2 0 0 3 ;2 0 0 4)。

语种：中文

中文关键词：白皮松;遗传多样性;杂合度;天然群体;有效等位基因数;多态位点;保育;表型多样性;松属;物种

外文关键词：Pinus bungeana; allozyme; genetic diversity; conservation strategy

分类号：S791.243;S826

摘要：在白皮松天然林中采取分层取样 ,抽取 10个群体 2 10个单株。测定分析 16种酶系统 ,得到 31个酶位点。白皮松物种水平遗传多样性参数为 :平均等位基因数AS=1 74 2 ,平均有效等位基因数AeS=1 4 9,多态位点百分率PS=5 4 8% ,期望杂合度HeS=0 16 2 ;白皮松群体水平遗传多样性参数为 :平均等位基因数AP=1 39± 0 11,平均有效等位基因数AeP=1 30± 0 12 ,多态位点百分率PP=34 85 %± 8 4 6 % ,期望杂合度HeP=0 0 99± 0 0 4 9,观测杂合度HoP =0 0 95± 0 0 4 2。白皮松遗传多样性在松属树种中属于中等偏下 ,群体间分化明显 ,遗传分化系数FST =0 133,群体间遗传距离 D =0 12 8,大于松属平均水平。根据Nei’s遗传一致度将 10个群体分为 3组。白皮松群体遗传多样性中心与资源分布中心和表型多样性分布中心吻合 ,适宜采取中心群体原地保护和多群体异地联合保存相结合的保护策略。
Ten populations with 210 families of Pinus bungeana were collected in its natural stands by delamination sampling method. Sixteen enzyme systems had been analyzed and 31 loci were measured. The results showed that the genetic diversity of P. bungeana was slight lower than the average of Pinus species. The parameters of genetic diversity of P. bungeana at species level were given as follows: mean number of alleles per locus A S=1.742, mean number of effective alleles per locus Ae S=1.49, percentage of polymorphic loci P S=54.8%, Hardy-weinberg expected heterozygosity He S=0.162. The parameters of genetic diversity of P. bungeana at population level were given as follows: A P=1.39±0.11, Ae P=1.30±0.12, P P=34.85%±8.46%, He P=0.099±0.049,directly counted mean heterozygosity Ho P=0.095±0.042. The genetic differentiation among populations of P. bungeana was obvious(F ST=0.133). The genetic distance between populations was large(=0.128). Ten populations were divided into three groups according to Nei's genetic identity. The genetic diversity of natural population of P. bungeana showed as central distribution, which was accordant with its natural resources distribution and its morphology diversity distribution, and its implication for conservation strategy was discussed.