文献类型：期刊文献

英文题名：Transcriptomic analysis reveals the significant effects of fertilization on the biosynthesis of sesquiterpenes in Phoebe bournei

作者：Liu, Li[1] Wang, Xu[2] Lai, Yong[3] He, Gongxiu[1] Wen, Shizhi[1] He, Hanjie[4] Li, Zhenshan[1] Zhang, Baohong[5] Zhang, Dangquan[3]

第一作者：Liu, Li

通信作者：He, GX[1];Zhang, DQ[2];Zhang, BH[3]

机构：[1]Cent South Univ Forestry & Technol, Sch Forestry, Changsha 410004, Peoples R China;[2]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[3]Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Peoples R China;[4]Cent South Univ Forestry & Technol, Sch Life Sci & Technol, Changsha 410004, Peoples R China;[5]East Carolina Univ, Dept Biol, Greenville, NC 27858 USA

年份：2022

卷号：114

期号：3

外文期刊名：GENOMICS

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000797865900002)】；

基金：We thank Professor Zhiming Liu and Dr. Dan Cao for their revisions to the manuscript and Nature Research Editing Service for help with English usage. This research was supported by the Natural Science Foundation of China (32071752 and 32171835) and the HunanProvincial Education Department, China (17A226) . This project was also supported by the Science and Technology Program of Changsha City (kq2005002) . The funders had no role in study design, data collection, analysis, decision to publish, or manuscript preparation.

语种：英文

外文关键词：Sesquiterpenes; Compost; Compound fertilizer; Biosynthesis pathway; DEG expression

摘要：Phoebe bournei is a potential medicinal plant. Its essential oils (Eos) are mainly composed of sesquiterpenes that has potential activities of anti-bacteria and anti-tumors. In this study, we evaluated the effects of compost and compound fertilizer on the total amount and main components of Eos in P. bournei, we also studied the molecular mechanism undergoing this process by deep sequencing the genes involved in the biosynthesis of sesquiterpenes. Fertilization enhanced the total amount of main components in Eos from both leaves and twigs. Bicyclo-germacrene, the primary sesquiterpene in the leaf EO, was significantly increased under compost treatment, while bicyclogermacrene and delta-cadinene (the second most abundant sesquiterpene) were decreased under compound fertilizer treatment. The two fertilizers had no significant effect on the abundance of the primary (+)-delta-cadinene in the twig EO, but had a positive effect on the second most abundant sesquiterpene copaene. Sig-nificant differences were observed in the number of differentially expressed genes (DEGs) with the leaves showing greater number of DEGs as compared to the twigs after compost treatment. Terpenoid backbone biosynthesis (TBB) is a key pathway of sesquiterpenes synthesis. The expression of genes regulating several important enzymes in TBB was altered after fertilization. After the compost treatment, the expression of the leaf DXS gene (ACQ66107.1), being closely related to the sesquiterpene biosynthesis in P. bournei leaves, was decreased. Compost and compound fertilizer altered the expression of the two important branch-point enzymes (FPPS and GGPPS) genes (ART33314.1 and ATT59265.1), which contributed to the changes of the total amount and components of P. bournei sesquiterpenes. This study provides a new insight into the future use of P. bournei for Eos.