文献类型：期刊文献

英文题名：A GH3-like gene, LaGH3, isolated from hybrid larch (Larix leptolepis × Larix olgensis) is regulated by auxin and abscisic acid during somatic embryogenesis

作者：Zhang, Li-feng[1,2] Lan, Qian[3] Han, Su-ying[4] Qi, Li-wang[1,2]

第一作者：张立峰

通信作者：Zhang, Li-feng

机构：[1] State Key Laboratory of Tree Genetics and Breeding, Research Institute of Forestry, Chinese Academy of Forestry, Beijing, 100091, China; [2] Key Laboratory of Tree Breeding and Cultivation, Research Institute of Forestry, National Forestry and Grassland Administration, Chinese Academy of Forestry, Beijing, 100091, China; [3] Research Institute of Forestry Policy and Information, Chinese Academy of Forestry, Beijing, 100091, China; [4] Research Institute of Forest Ecology, Environment and Protection, Chinese Academy of Forestry, Beijing, 100091, China

年份：2019

卷号：33

期号：6

起止页码：1723-1732

外文期刊名：Trees - Structure and Function

收录：EI(收录号：20193707431709);Scopus(收录号：2-s2.0-85072023337)

基金：This work was supported by the National Natural Science Foundation of China (31600544 and 31330017), the Fundamental Research Funds for the Central Non-profit Research Institution of CAF (CAFYBB2019SY011), and the National Transgenic Major Program of China (2018ZX08020-003).

语种：英文

外文关键词：Acetic acid - Animal cell culture - Cloning - Genes - pH - Polycyclic aromatic hydrocarbons - Polymerase chain reaction - Proteins

摘要：Key message: A Gretchen Hagen 3-like gene, LaGH3 from hybrid larch (Larix leptolepis × Larix olgensis), was identified, and the effects of abscisic acid (ABA) and indole-3-acetic acid (IAA) on its transcriptional levels and expression patterns during somatic embryogenesis were analyzed. Abstract: A full-length cDNA sequence of a Gretchen Hagen 3 (GH3) gene was isolated from hybrid larch (Larix leptolepis × Larix olgensis) and designated LaGH3. The cDNA was 2053bp in length and contained an 1848-bp open-reading frame encoding a predicted protein of 615 amino acids, characterized by four conserved motifs of the GH3 protein family: P-loop, α5, α6, and β8–β9. The 5′-flanking promoter region of LaGH3 was cloned using an improved TAIL-PCR technique. In this region, we identified several auxin- and abscisic acid (ABA)-inducible elements. Expression analysis showed that LaGH3 was induced by indole-3-acetic acid and that its transcript accumulation was inhibited by ABA. Further experiments demonstrated that ABA was the main factor in LaGH3 downregulation during the early stage of somatic embryogenesis, although the absence of auxin decreased LaGH3 expression slightly. LaGH3 transcripts gradually increased and peaked at 28 days with the rapid development of somatic embryos and then declined as the somatic embryos matured. We suggest that the level of LaGH3 transcripts partly represents the dynamics of endogenous auxin during somatic embryo development in larch. Taken together, our results provide evidence that GH3 genes play important roles in the crosstalk between auxin and ABA by maintaining auxin homeostasis during somatic embryo development. ? 2019, Springer-Verlag GmbH Germany, part of Springer Nature.