文献类型：期刊文献

中文题名：三元低共熔溶剂快速解离毛竹及提高酶水解得率研究

英文题名：Rapid fractionation of moso bamboo for enhancing enzymatic saccharification yield using ternary deep eutectic solvent

作者：陈婷珺[1] 周雪莲[1,2] 詹云妮[1] 刘旭泽[1] 黄晨[1] 邓拥军[1,2] 房桂干[1,2]

第一作者：陈婷珺

机构：[1]中国林业科学研究院林产化学工业研究所,江苏省生物质能源与材料重点实验室,南京210042;[2]江苏省林业资源高效加工利用协同创新中心,南京210037

年份：2023

卷号：8

期号：5

起止页码：86-92

中文期刊名：林业工程学报

外文期刊名：Journal of Forestry Engineering

收录：CSTPCD;;Scopus;北大核心:【北大核心2020】；CSCD:【CSCD_E2023_2024】；

基金：江苏省生物质能源与材料重点实验室基本科研业务费项目(JSBEM-S-202203)。

语种：中文

中文关键词：毛竹;低共熔溶剂(DES);快速解离;酶水解;高纯度木质素

外文关键词：moso bamboo;deep eutectic solvent;rapid fractionation;enzymatic hydrolysis;high purity lignin

分类号：O636.2

摘要：以速生毛竹为原料,采用AlCl 3辅助的多元醇基低共熔溶剂(氯化胆碱/1,4-丁二醇/AlCl 3)进行预处理,实现毛竹组分温和、快速分离,并借助扫描电子显微镜(SEM)、X射线衍射(XRD)等手段考察低共熔溶剂(DES)预处理对纤维素酶水解的促进机制。研究表明:氯化胆碱/1,4-丁二醇/AlCl 3体系可在10~30 min大量脱除木质素和木聚糖,同时保留绝大部分纤维素;预处理物料纤维素酶水解得率均超过89%,溶解木质素可通过简单方式回收获得高纯度木质素,回收率超过90%。SEM结果显示,预处理后纤维表面出现明显的断裂和团聚,表明该体系对毛竹纤维的润涨断裂效果较强;XRD分析表明,经过该DES体系预处理60 min后,纤维素结晶度由原料的59.59%增加至63.28%,而聚合度则由原料的773显著下降至341。在DES预处理过程中,木质素和半纤维素的大量脱除以及纤维的碎片化显著增加了酶与纤维素的接触,大幅提高了纤维素的糖化得率,且分离的木质素纯度高达96.34%。因此,氯化胆碱/1,4-丁二醇/AlCl 3体系是一种快速、高效的预处理体系,可在提高预处理物料纤维素酶解得率的同时分离得到高得率、高纯度的木质素,进一步实现高值化利用。
Deep eutectic solvents(DES)composed of hydrogen bond donor and hydrogen bond acceptor have been widely used for lignocellulose pretreatment because of its easy preparation,biodegradability,environment friendliness,low cost and nonflammability.In this study,rapid and gentle fractionation of moso bamboo was achieved using AlCl 3-assisted polyol deep eutectic solvent(choline chloride/1,4-butaneol/AlCl 3)as pretreatment medium.The mechanism of DES pretreatment on cellulose hydrolysis was investigated by scanning electron microscopy(SEM)and X-ray diffractometry(XRD).The results showed that the system can achieve effective fractionation of bamboo within 30 min,resulting in>61% lignin removal,>84% xylan removal and>90% glucan retention.Specifically,after 10 min pretreatment,the DES system could result in 61.34% lignin removal and 84.15% xylan removal,indicating that the short-time fractionation could lead to satisfying fractionation performance.In addition,the near theoretical glucan could be recovered,indicating the resistance of glucan to the DES.Remarkably,the glucan enzymatic hydrolysis yield was all beyond 89%,and the lignin recovery yield could reach over 90% with nearly no carbohydrates.The significant enhancement of the enzymatic hydrolysis yield was mainly ascribed to the significant lignin removal which greatly reduced bamboo recalcitrance,and the fit index between the lignin removal and enzymatic hydrolysis yield reached as high as 96.2%.The SEM results showed that the fiber surface of the pretreated substrates was obviously fractured and agglomerated,indicating the effective swelling and degradation effect by the DES system.The shortened and fractured fibers of the pretreated bamboo feedstock could efficiently facilitate the subsequent enzymatic hydrolysis by increasing the contact area between cellulase and cellulose.The X-ray diffraction result showed that the CrI of the cellulose increased from 59.59% to 63.28%,while the cellulose DP decreased from 773 to 341.The increased CrI was mainly induced by the removal of the amorphous xylan and lignin,while the DP decrease was resulted from the cellulose fracture during the fractionation process.It should be noted that the enhanced CrI and decreased cellulose DP were also the main promoter for the enzymatic hydrolysis process.Notably,the proposed DES system could obtain an excellent lignin recovery yield which is an essential prerequisite for the lignin valorization.In addition,the isolated lignin featured a 96.34% purity,suggesting that the DES system could obtain a valuable lignin with high yield and high purity.Therefore,choline chloride/1,4-butaneol/AlCl_(3) is an efficient pretreatment system which could yield high cellulose saccharification and obtain lignin with high yield and high purity for its further valorization.