文献类型：期刊文献

英文题名：Novel insights on genes and pathways involved in Pinus elliottii response to resinosis

作者：Zhang, Guoyun[1] Zhang, Xu[2] Yu, Sujun[3] Sun, Honggang[2]

第一作者：张国昀

通信作者：Sun, HG[1]

机构：[1]Chinese Acad Forestry, Res Inst Forestry, Beijing 100091, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Hangzhou 311400, Zhejiang, Peoples R China;[3]Fengshushan Forestry Farm, Jingdezhen 333000, Jiangxi, Peoples R China

年份：0

外文期刊名：TREE PHYSIOLOGY

收录：;Scopus(收录号：2-s2.0-85147893441);WOS:【SCI-EXPANDED(收录号:WOS:000891544900001)】；

语种：英文

外文关键词：degradome sequencing; leaf; RNA-seq; SMRT

摘要：Pinus elliottii, an important coniferous timber species, has recently become one of the most popular sources of resin in China. Resinosis is a common disease that may negatively affect pine tree growth and production. In this study, we used single-molecule real-time sequencing and Illumina RNA sequencing to generate an accurate transcriptome for P. elliottii. The transcriptome included 90,026 transcripts, 5160 long non-coding RNAs and 7710 transcription factors. We then analyzed RNA-sequencing, small RNA-sequencing and degradome data to identify genes, miRNAs and key miRNA-target pairs involved in response to resinosis in P. elliottii. We identified 1305 genes and 1151 miRNAs exhibiting significant differential expression in response to resinosis. According to the degradome sequencing analysis, 318 differentially expressed transcripts were targets of 14 differentially expressed miRNAs. Our study has provided resources for further functional characterization of genes and miRNAs involved in resinosis in P. elliottii, which should aid the future disease-resistance breeding of this species.