文献类型：期刊文献

英文题名：The yellowhorn AGL transcription factor gene XsAGL22 contributes to ABA biosynthesis and drought tolerance in poplar

作者：Bian, Zhan[1] Wang, Xiaoling[2] Lu, Junkun[1] Wang, Dongli[1] Zhou, Yangyan[3] Liu, Yunshan[4] Wang, Shengkun[1] Yu, Zequn[5] Xu, Daping[1] Meng, Sen[1,4]

第一作者：Bian, Zhan

通信作者：Meng, S[1];Meng, S[2]

机构：[1]Chinese Acad Forestry, Res Inst Trop Forestry, State Key Lab Tree Genet & Breeding, Guangzhou 510520, Guangdong, Peoples R China;[2]Jiangxi Acad Sci, Inst Biol Resources, Nanchang 330096, Jiangxi, Peoples R China;[3]Salver Acad Bot, Rizhao 262300, Shandong, Peoples R China;[4]Chongqing Three Gorges Univ, Coll Biol & Food Engn, Chongqing 404100, Peoples R China;[5]Shanghai Gardening Landscaping Construct Co Ltd, Shanghai 200333, Peoples R China

年份：0

外文期刊名：TREE PHYSIOLOGY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000787172700001)】；

基金：This research was supported by grants from the Fundamental Research Fund sfor the Central Non- profit Research Institution of CAF[ CAFYBB2019QD001 and CAFYBB2020SY018], the National Natural Science Foundation of China [ 31901304] and the Natural Science Foundation of Guangdong Province, China [ 2019A1515011595].

语种：英文

外文关键词：ABA biosynthesis; drought resistance; XsAGL22; XsBG1; XsNCED6; yellowhorn

摘要：Regulation of abscisic acid (ABA) biosynthesis helps plants adapt to drought stress, but the underlying molecular mechanisms are largely unclear. Here, a drought-induced transcription factor XsAGL22 was isolated from yellowhorn (Xanthoceras sorbifolium Bunge). Yeast one-hybrid and electrophoretic mobility shift assays indicated that XsAGL22 can physically bind to the promoters of the ABA biosynthesis-related genes XsNCED6 and XsBG1, and a dual-luciferase assay showed that XsAGL22 activates the promoters of the later two genes. Transient overexpression of XsAGL22 in yellowhorn leaves also increased the expression of XsNCED6 and XsBG1 and increased cellular ABA levels. Finally, heterologous overexpression of XsAGL22 in poplar increased ABA content, reduced stomatal aperture and increased drought resistance. Our results suggest that XsAGL22 is a powerful regulator of ABA biosynthesis and plays a critical role in drought resistance in plants.