文献类型：期刊文献

中文题名：亚临界水提取协同大孔树脂纯化杨树芽总黄酮

英文题名：Extraction and Purification of Flavonoids from Poplar Buds with Subcritical Water Extraction Coordinated with Macroporous Resin Adsorption

作者：薄采颖[1,2,3,4] 郑光耀[1,2,3,4] 高丽萍[1,2,3,4] 张建华[5] 戴海静[5]

第一作者：薄采颖

通信作者：Zheng, Guang-Yao

机构：[1]中国林业科学研究院林产化学工业研究所;[2]生物质化学利用国家工程实验室;[3]国家林业局林产化学工程重点开放性实验室;[4]江苏省生物质能源与材料重点实验室;[5]江苏华安科研仪器有限公司

年份：2014

卷号：34

期号：4

起止页码：107-112

中文期刊名：林产化学与工业

外文期刊名：Chemistry and Industry of Forest Products

收录：CSTPCD;;EI(收录号：20143900069351);Scopus(收录号：2-s2.0-84907098367);北大核心:【北大核心2011】；CSCD:【CSCD2013_2014】；

基金：江苏省产学研前瞻性联合研究项目(BY2011116)

语种：中文

中文关键词：杨树芽;亚临界水;总黄酮;大孔树脂

外文关键词：poplar buds ; subcritical water; flavonoids ; macroporous adsorption resin

分类号：TQ35

摘要：采用亚临界水提取-大孔树脂纯化联用技术对杨树芽中总黄酮进行提取、纯化,以提取温度、液料比和提取时间为考察因素,以总黄酮的得率为考察指标,采用正交试验优化亚临界水对杨树芽总黄酮的提取工艺;从4种大孔吸附树脂中筛选出对总黄酮有最佳分离纯化效果的一种树脂,研究其对总黄酮静态和动态吸咐以及解吸效果.结果表明,5 MPa下亚临界水提取杨树芽总黄酮的最佳工艺条件为：提取温度180℃、提取时间10 min、液料比30∶ 1（mL∶g）,总黄酮的粗品提取得率为11.83％,纯度为13.16％.通过静态吸附试验筛选出最有效的HP-20树脂分离纯化杨树芽总黄酮,通过动态吸附试验确定应用HP-20树脂吸附分离杨树芽总黄酮的最佳工艺条件为：上样质量浓度为4 g/L,流速为60 mL/h,pH值为2～3,此时吸附量为42.69 g/L洗脱剂乙醇的体积分数为90％,解吸率为93.91％,经纯化后总黄酮的纯度为49.28％.
Subcritical water extraction coupled with macroporous adsorption resin technology was applied to extract and purify flavonoids from poplar buds. Based on the yield of flavonoids, the factors of extraction technology including extraction temperature, liquid-feed ratio was optimized through orthogonal tests. By comparison of four macroporous resins in the adsorption of flavonoids, the optimal macroporous of resin was selected, then adsorption and desorption effects were investigated. The results showed that the optimum process of subcritical water extraction were extraction temperature 180 ℃, liquid-feed ratio 30 ： l, extraction time 10 rain. The yield of crude flavonoids was 11.83 % and the purity of the product is 13. 16%. The HP-20 macroporous adsorption resin was selected to separate and purify flavonoids from poplar buds. The optimal technological conditions were solution concentration 4 g/L, flow rate 60 mL/h, solution pH 2 - 3, and eluting solvent 90 % ethanol. The purity of flavonoids can reach 49.28 %.