文献类型：期刊文献

中文题名：南方红豆杉人工引种及野生植株活性成分含量与分布

英文题名：Content and distribution of active components in cultivated and wild Taxus chinensis var.mairei plants

作者：于少帅[1] 孙启武[2] 张小平[1] 田胜尼[3] 柏培磊[1]

第一作者：于少帅

机构：[1]安徽师范大学生命科学学院/安徽省重要生物资源保护与利用研究重点实验室/生物环境与生态安全安徽省重点实验室;[2]中国林业科学研究院林业研究所/国家林业局林木培育重点实验室;[3]安徽农业大学生命科学学院

年份：2012

卷号：23

期号：10

起止页码：2641-2647

中文期刊名：应用生态学报

外文期刊名：Chinese Journal of Applied Ecology

收录：MEDLINE(收录号：23359921);CSTPCD;;Scopus(收录号：2-s2.0-84868222292);北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；PubMed;

基金：国家财政专项项目(2130211);国家自然科学基金项目(30840020;30970292)资助

语种：中文

中文关键词：南方红豆杉;活性成分;人工栽培;紫杉醇

外文关键词：Taxus chinensis var.mairei; active component; artificial cultivation; paclitaxel.

分类号：S791.49

摘要：南方红豆杉是我国特有的濒危植物,因其具有抗癌药用价值而被过度采集,导致现存种群减少.采用超声辅助提取和HPLC检测人工引种及野生南方红豆杉植株中紫杉醇、7-木糖紫杉醇、三尖杉宁碱等活性成分含量,分析其在不同部位、年限和坡向的分布规律.结果表明:南方红豆杉人工引种后,植株中的紫杉醇、7-木糖紫杉醇和三尖杉宁碱含量变化明显,野生植株中紫杉醇含量显著高于人工栽培植株,约为栽培植株的1.8倍,其他2种物质含量以人工栽培略高.不同部位、冠层样品中3种活性物质含量差异显著,以茎皮、高冠层枝叶含量较高.不同年限南方红豆杉植株中紫杉醇、7-木糖紫杉醇和三尖杉宁碱含量以4年生植株较高,分别为0.08、0.91和0.32mg·g-1.不同坡向植株中3种活性成分含量以阳坡植株较高,紫杉醇和7-木糖紫杉醇含量差异显著,三尖杉宁碱含量差异不显著.南方红豆杉中3种活性成分的积累与光照条件密切相关,栽培过程中适当增加光照有利于3种活性成分的积累.
Taxus chinensis var.mairei is an endemic and endangered plant species in China.The resources of T.chinensis var.mairei have been excessively exploited due to its anti-cancer potential,accordingly,the extant T.chinensis var.mairei population is decreasing.In this paper,ultrasonic extraction and HPLC were adopted to determine the contents of active components paclitaxel,7-xylosyltaxol and cephalomannine in cultivated and wild T.chinensis var.mairei plants,with the content distribution of these components in different parts of the plants having grown for different years and at different slope aspects investigated.There existed obvious differences in the contents of these active components between cultivated and wild T.chinensis var.mairei plants.The paclitaxel content in the wild plants was about 0.78 times more than that in the cultivated plants,whereas the 7-xylosyltaxol and cephalomannine contents were slishtly higher in the cultivated plants.The differences in the three active components contents between different parts and tree canopies of the plants were notable,being higher in barks and upper tree canopies.Four-year old plants had comparatively higher contents of paclitaxel,7-xylosyltaxol and cephalomannine （0.08,0.91 and 0.32 mg·g-1,respectively）,and the plants growing at sunny slope had higher contents of the three active components,with significant differences in the paclitaxel and 7-xylosyltaxol contents and unapparent difference in the cephalomannine content of the plants at shady slope.It was suggested that the accumulation of the three active components in T.chinensis var.mairei plants were closely related to the sunshine conditions.To appropriately increase the sunshine during the artificial cultivation of T.chinensis var.mairei would be beneficial to the accumulation of the three active components in T.chinensis var.mairei plants.