文献类型：期刊文献

英文题名：PzTAC and PzLAZY from a narrow-crown poplar contribute to regulation of branch angles

作者：Xu, Dong[1] Qi, Xiao[1] Li, Jihong[1] Han, Xiaojiao[2] Wang, Jinnan[1] Jiang, Yuezhong[3] Tian, Yanting[1] Wang, Yiwei[1]

第一作者：Xu, Dong

通信作者：Li, JH[1];Han, XJ[2]

机构：[1]Shandong Agr Univ, Coll Forestry, Key Lab Agr Ecol & Environm, Tai An 271018, Shandong, Peoples R China;[2]Chinese Acad Forestry, Res Inst Subtrop Forestry, Key Lab Tree Breeding Zhejiang Prov, Hangzhou 311400, Zhejiang, Peoples R China;[3]Forestry Sci Acad Shandong, Shandong Prov Key Lab Forest Tree Genet Improveme, Jinan 250014, Shandong, Peoples R China

年份：2017

卷号：118

起止页码：571-578

外文期刊名：PLANT PHYSIOLOGY AND BIOCHEMISTRY

收录：;WOS:【SCI-EXPANDED(收录号:WOS:000411169500058)】；

基金：This study was supported by the National High Technology Research and Development Program of China (no. 2013AA102703), Natural Science Foundation of Shandong Province of China (no. ZR2013CM020), and Agricultural Breeding Program of Shandong Province of China (201496).

语种：英文

外文关键词：PzTAC; PzLAZY; Poplar; Branch angle; Narrow-crown

摘要：Plant architecture, as a basic element influenced by genetic and environmental factors, has an important effect on grain yield via light transmission in agroforestry systems. The molecular mechanism underlying control of branch angle, an important aspect of tree architecture, is not well understood in poplars. Here, we cloned two genes from Populus x zhaiguanheibaiyang (a narrow-crown poplar), designated PzTAC and PzLAZY, which were predicted to be members of the ITG gene family through sequence homology. Transcript levels of the homologous genes were estimated by reverse transcriptase quantitative PCR (RT-qPCR) in different organs of P. x zhaiguanheibaiyang and P. Deltoides 'Zhonglin2025' (a broad-crown poplar). TAC expression was mainly confined to the leaves and annual shoots, whereas LAZY was mainly expressed in the annual shoots and axillary buds. Beside, we detected the promoter expression patterns derived from the PzTAC and PzLAZY genes using the beta-glucuronidase (GUS) reporter gene in transgenic Populus x euramericana 'Neva'. GUS activity driven by the PzTAC and PzLAZY promoters was detected in mature leaves, leaf axils and vascular tissues of roots. The PzTAC promoter was mainly active in leaf veins, whereas the PzLAZY promoter was mainly active in mesophyll cells and root tips. The average branch angle in transgenic 35S::PzTAC plants was larger than that of transgenic 35S::PzLAZY plants. The results provide strong evidence that the two genes affect the vascular tissues of transgenic plants to modify branch angles. (C) 2017 Published by Elsevier Masson SAS.