文献类型：期刊文献

中文题名：冷锻炼处理减轻低温贮藏桃果实冷害的能量代谢机理

英文题名：Mechanism of energy metabolism on cold acclimation treatment for alleviating chilling injury of peach fruit during low temperature storage

作者：祝美云[1] 白欢[1,2] 梁丽松[2] 王贵禧[2]

第一作者：祝美云

通信作者：Wang, G.

机构：[1]河南农业大学食品科学技术学院;[2]中国林业科学研究院林业研究所国家林业局林木培育实验室

年份：2012

卷号：28

期号：23

起止页码：257-264

中文期刊名：农业工程学报

外文期刊名：Transactions of the Chinese Society of Agricultural Engineering

收录：CSTPCD;;EI(收录号：20130315914970);Scopus(收录号：2-s2.0-84872245980);北大核心:【北大核心2011】；CSCD:【CSCD2011_2012】；

基金：2010年度国家自然科学基金(31071834)

语种：中文

中文关键词：果实;贮藏;酶;桃;冷锻炼处理;ATP

外文关键词：fruits, storage, enzymes, peach, cold acclimation treatment, ATP

分类号：S609.3;S662.1

摘要：为探讨通过维持低温贮藏桃果实能量代谢水平而减轻冷害的有效方法,以‘大久保’桃为试材,分别以24、5、0℃贮藏为对照,研究冷锻炼处理(8℃冷锻炼5d后转入0℃冷藏,CAT)对维持冷藏及其货架期间桃果实线粒体呼吸代谢相关酶及三磷酸腺苷(ATP)含量的影响。结果表明:24℃贮藏模式下桃果实的H+-ATPase、Ca2+-ATPase、琥珀酸脱氢酶(SDH)和细胞色素氧化酶(CCO)活性在贮藏过程中2～3d出现了活性高峰,之后随着果实的软化衰老而显著下降。5℃贮藏桃果实贮藏期间的H+-ATPase、Ca2+-ATPase、SDH、CCO活性和ATP含量最低,且下降速度也最快;0℃贮藏次之,而CAT贮藏可有效抑制桃果实H+-ATPase、SDH和CCO活性的下降,保持较高的ATP含量。随着贮藏时间的延长,货架期期间H+-ATPase、Ca2+-ATPase、SDH、CCO活性和ATP含量均降低,但CAT处理明显高于5、0℃冷藏处理。这表明冷锻炼处理可通过调节线粒体呼吸代谢酶活性,维持果实较高的能量水平,有利于减轻果实冷害的发生并延长贮藏期。
To investigate the effective ways of reducing the chilling injury of peach fruits by maintaining energy metabolism level at low temperature storage, the ＇Okubo＇ peach fruits were pre-stored at 8℃ for 5 d and then stored at 0℃ for 60 d （cold acclimation treatment, CAT）, and 24℃, 5℃, 0℃ storage were as CKs. All of fruits were packed （6-40/bag） with polyethylene plastic（0.04 mm, 10 holes） and the pocket felled with no tying to ensure gas exchange. Samples were measured every day for 24℃, 5 d for 5℃, 10 d for 0℃and CAT. Each time 40 fruits （4-10/bag） were taken completely random and divided into two parts, 20 fruits were measured directly and the other 20 fruits were placed for 3 d shelf-life under 24℃. The effect of CAT on mitochondria respiratory metabolism-related enzymes and ATP contents during storage and shelf-life were investigated. The results showed that the H^＋-ATPase, Ca^2＋-ATPase, SDH and CCO activities of peach fruits appeared active peaks at 2-3 d during 24℃ storage, then significantly decreased with the softening of fruit. The activities of H^＋-ATPase, Ca^2＋-ATPase, SDH,CCO and ATP contents of 5 ℃ treatment are lowest, and their decrease speed are the fastest under this condition. 0℃ storage has better effect than 5℃, while CAT storage can effectively inhibit the reduction of H^＋-ATPase, SDH and CCO activities, and maintain higher contents of ATP. With the storage duration, the H^＋-ATPase, Ca^2＋-ATPase, SDH and CCO activities and ATP contents all decreased during shelf-life, but CAT treatment was significantly better than 5 ℃ and 0 ℃ processing. These results suggest that CAT treatment may maintain high energy level by regulating the activities of mitochondria respiratory metabolism-related enzymes, thereby prevent the development of chilling injury of cold-stored peach fruit and prolong storage period.