文献类型：期刊文献

英文题名：The telomere-to-telomere (T2T) genome provides insights into the evolution of specialized centromere sequences in sandalwood

作者：Peng, Dan[1,2] Hong, Zhou[3] Kan, Shenglong[4] Wu, Zhiqiang[1] Liao, Xuezhu[1]

第一作者：Peng, Dan

通信作者：Wu, ZQ[1];Liao, XZ[1]

机构：[1]Chinese Acad Agr Sci, Agr Genom Inst Shenzhen, Shenzhen Branch,Lab Minist Agr & Rural Affairs, Guangdong Lab Lingnan Modern Agr,Key Lab Synthet B, Shenzhen 518120, Peoples R China;[2]Fujian Agr & Forestry Univ, Haixia Inst Sci & Technol, Ctr Genom & Biotechnol, Fuzhou 350002, Peoples R China;[3]Chinese Acad Forestry, Res Inst Trop Forestry, Guangzhou 510520, Peoples R China;[4]Shandong Univ, Marine Coll, Weihai 264209, Peoples R China

年份：2024

卷号：13

外文期刊名：GIGASCIENCE

收录：;Scopus(收录号：2-s2.0-85212459595);WOS:【SCI-EXPANDED(收录号:WOS:001374284100001)】；

基金：This work was funded by the Guangdong Pearl River Talent Program (grant 2021QN02N792), the Chinese Academy of Agricultural Sciences Elite Youth Program(grant 110243160001007), and the Shenzhen Fundamental Research Program (grant JCYJ20220818103212025).

语种：英文

外文关键词：T2T genome; centromere; hemiparasitic species; cyto-nuclear transfer

摘要：Background Sandalwood, a prized hemiparasitic plant, is highly sought in the commercial market because of its aromatic core materia. The structure and stability of the genome are instrumental in the rapid adaptation of parasitic plants to their surroundings. However, there is a conspicuous lack of research on the genomic-level adaptive evolution of sandalwood.Results In this study, we assembled a gap-free telomere-to-telomere (T2T) reference genome for Santalum album using PacBio HiFi, Hi-C, and ultra-long ONT data. The T2T reference genome (Sal_t2t) encompassed annotations of 24,171 genes and 25.34% repetitive sequences, in addition to all 10 centromeres and 20 telomeres across the 10 chromosomes. The results revealed that the 3 distinct parasitic species of Santalales had diverse centromeric compositions. The Copia-type long terminal repeat transposon emerged as the most significant in the S. album genome, constituting the primary sequence of the centromere and influencing gene expression. Third, in sandalwood, the presence of Copia affected the size of the centromeres and, consequently, the genome size. Identification of the sandalwood T2T genome in this study also enabled the identification of more precise organelle transfer fragments.Conclusions Our research provides a sandalwood T2T genome, laying the groundwork for future investigations on the evolution of energy organs in parasitic plants. Moreover, it offers novel insights into the function and evolution of centromeres, as well as the mechanisms of adaptation and parasitism.