文献类型：期刊文献

中文题名：漆树组织培养中污染与褐变的防治

英文题名：Study on the Prevention and Cure of Browning and Contamination During Tissue Culture of Toxicodendron vernicifluum

作者：赵月明[1] 赵雁鸣[1] 何承忠[1] 廖声熙[2] 李旦[3]

第一作者：赵月明

机构：[1]西南林业大学/西南山地森林资源保育与利用省部共建教育部重点实验室;[2]中国林业科学研究院资源昆虫研究所;[3]西南林业大学/云南生物多样性研究院

年份：2015

卷号：31

期号：10

起止页码：33-38

中文期刊名：中国农学通报

外文期刊名：Chinese Agricultural Science Bulletin

收录：CSTPCD

基金：云南省教育厅重大专项"优良小木漆组培快繁技术及多倍体育种研究"(ZD2013014);云南省学术与技术带头人后备人才培养基金项目(2012HB021);西南林业大学科研启动基金资助项目"优良小木漆组培扩繁研究"(111409)

语种：中文

中文关键词：漆树;组织培养;污染;褐变

外文关键词：Toxicodendron vernicifluum; tissue culture; contamination; browning

分类号：S722.37

摘要：漆树作为重要的经济树种,可通过植物组织培养扩繁,但在其组织培养过程中会出现严重的污染和褐变。本研究使用制霉菌素、两性霉素B、苯扎氯胺,乙醇和升汞克服污染;使用半胱氨酸,MS和White培养基克服褐变。用SPSS 12.0统计软件进行单因素方差分析和Duncan新复极差法多重比较分析,通过统计污染率、褐变率和成活率,得出最佳消毒方法以75%乙醇浸泡1 min,1%苯扎氯胺浸泡1 min,0.1%升汞浸泡20 min效果最好,污染率为1.15%,并得出最佳防治褐变培养基以添加100 mg/L半胱氨酸的White培养基效果最好,褐变率为1.15%,并将成活率提高到97.63%。试验所建立的漆树高效、稳定的无菌体系,为后期其完整的植物组织培养体系的建立奠定了基础。
As an important economic species, Toxicodendron vernicifluum could be propagated by the way ofplant tissue culture. In order to solve the contamination, nystatin, amphotericin B, benzalkonium chloride, 75%ethanol and 0.1% mercuric chloride were used. Besides, cysteine, MS and White medium were used to solvebrowning. SPSS 12.0 statistical software was used for the single factor analysis of variance and Duncan＇s newmultiple comparison analysis. By counting contamination rate, browning rate and survival rate, the best methodof disinfection was 75% ethanol soaked 1 min, 1% benzalkonium soak 1 min, 0.1% mercuric chloride for20 min, and the contamination rate was 1.15%. The best optimum medium was White culture mixed with100 mg/L cysteine, and the browning rate was 1.15%. The survival rate was improved to 97.67%. The efficientand stable sterile system of Toxicodendron vernicifluum had been laid a solid foundation for the whole tissueculture system.