文献类型：期刊文献

英文题名：Characterization of hemicellulose in Cunninghamia lanceolata stem during xylogenesis

作者：Zheng, Biao[1,2] Yang, Haoqiang[1,2] Xu, Xiaoli[3] Xiang, Zhouyang[4] Hong, Zhou[5] Zheng, Huiquan[6] Wu, Aimin[1,2] Li, Huiling[1,2]

第一作者：Zheng, Biao

通信作者：Wu, AM[1];Li, HL[1];Zheng, HQ[2]

机构：[1]South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou 510642, Peoples R China;[2]South China Agr Univ, Coll Forestry & Landscape Architecture, Guangdong Key Lab Innovat Dev & Utilizat Forest Pl, Guangzhou 510642, Peoples R China;[3]South China Agr Univ, Instrumental Anal & Res Ctr, Guangzhou 510642, Peoples R China;[4]South China Univ Technol, State Key Lab Pulp & Paper Engn, Guangzhou 510640, Peoples R China;[5]Chinese Acad Forestry, Res Inst trop Forestry, Guangzhou 510520, Peoples R China;[6]Guangdong Acad Forestry, Guangdong Prov Key Lab Silviculture Protect & Util, Guangzhou 510520, Peoples R China

年份：2023

卷号：246

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20232814379449);Scopus(收录号：2-s2.0-85164222922);WOS:【SCI-EXPANDED(收录号:WOS:001038125900001)】；

基金：Financial support for this work was obtained Key -Area Research and Development Program of Guangdong Province (No.2020B020215001) , the National Natural Science Foundation of China (Grant Numbers 31870653) , Guangzhou Science and Technology Program key projects (Grant Numbers 201904020014) , and the Science and Technology Program of Guangzhou, China (202201011282) .

语种：英文

外文关键词：Cunninghamia lanceolata; Hemicellulose; Xylogenesis; Structural characterization

摘要：In this study, hemicellulose was isolated from the apical, middle and basal segments of C. lanceolata stem to investigate the dynamic change of its structure during xylogenesis. Results showed that the C. lanceolata hemicellulose is mainly consisted of O-acetylgalactoglucomannan (GGM) which backbone is alternately linked by & beta;-D-mannopyranosyl (Manp) and & beta;-D-glucopyranosyl (Glcp) via (1 & RARR; 4)-glycosidic bond, while the side chains are & alpha;-D-galactopyranosyl (Galp) and acetyl. In addition, 4-O-methylglucuronoarabinoxylan (GAX) is another dominant structure of C. lanceolata hemicellulose which contains a linear backbone of (1 & RARR; 4)-& beta;-D-xylopyranosyl (Xylp) and side chains of 4-O-Me-& alpha;-D-glucuronic acid (MeGlcpA) and & alpha;-L-arabinofuranose (Araf). The thickness of the cell wall, the ratio of GGM/GAX and the molecular weight of hemicellulose were increased as the extension of growth time. The degree of glycosyl substitutions of xylan and mannan was decreased from 10.34 % (apical) to 8.38 % (basal) and from 15.63 % (apical) to 10.49 % (basal), respectively. However, the total degree of acetylation was enhanced from 0.28 (apical) to 0.37 (basal). Transcriptome analysis showed that genes (CSLA9, IRX9H1, IRX10L, IRX15L, GMGT1, TBL19, TBL25, GUX2, GUX3, GXM1, F8H1 and F8H2) related to hemicellulose biosynthesis are mainly expressed in mature part. This study is of great significance for genetic breeding and high-value utilization of C. lanceolata.