文献类型：期刊文献

中文题名：基于HS-SPME和GC-MS联用技术测定不同种泡桐花挥发性成分

英文题名：Determination of volatile components in the flowers of different Paulownia species based on HS-SPME and GC-MS

作者：冯延芝[1,2,3] 杨超伟[1,2,3] 王保平[1,2,3] 乔杰[1,2,3] 周海江[1,2,3] 赵阳[1,2,3]

第一作者：冯延芝

机构：[1]中国林业科学研究院经济林研究所;[2]经济林种质创新与利用国家林业和草原局重点实验室;[3]泡桐国家创新联盟,郑州450003

年份：2023

卷号：35

期号：7

起止页码：1163-1171

中文期刊名：天然产物研究与开发

外文期刊名：Natural Product Research and Development

收录：CSTPCD;;CSCD:【CSCD2023_2024】；

基金：河南省科技兴林项目(YLK202213)。

语种：中文

中文关键词：泡桐;不同种类;花;顶空固相微萃取和气相色谱-质谱联用技术;挥发性成分

外文关键词：Paulownia;different species;flower;headspace solid-phase microextraction technology and gas chromatography-mass spectrometry;volatile components

分类号：S792.43;Q946.91

摘要：本文采用顶空固相微萃(HS-SPME)和气相色谱-质谱(GC-MS)联用技术测定白花泡桐、兰考泡桐、楸叶泡桐、毛泡桐和白花泡桐天然杂种花中的挥发性成分,分析不同种间挥发性成分组成和相对含量,并对其进行聚类和主成分分析。结果表明,5个不同种泡桐花中共鉴定出45种挥发性物质,主要包括12种萜类、8种醇类、7种酯类、3种酚类、6种醛类、2种木脂素、2种苯类、2种酮类、2种烷烃和1种醚类共10类物质;5个泡桐种共有挥发性组分7种,包括1种萜类、2种醇类、1种酯类、2种酚类、1种酮类,且其特有物质分别为9、1、4、3、0种;5个泡桐种均以醇类化合物的相对含量最高,且醇类化合物中均以1-辛烯-3-醇和3-辛醇的相对含量较高;聚类分析结果表明楸叶泡桐和毛泡桐相似性较高,而白花泡桐与其他种泡桐相似性均较低;第一主成分(PC1)和第二主成分(PC2)之和为70.7%,PC1的主要贡献物质为酯类、烷烃、萜类、醚类、酮类、醇类和苯类,PC2的主要贡献物质为酚类、醇类、醛类、酮类、醚类、苯类和木脂素;偏最小二乘法-判别分析(PLS-DA)筛选出1-辛烯-3-醇、1,2,4-三甲氧基苯和顺式茴香烯等11种标志性差异挥发性成分。本研究可为不同种泡桐花挥发性成分的精准评价及高效利用提供参考依据。
The headspace solid-phase microextraction technology(HS-SPME)and gas chromatography-mass spectrometry(GC-MS)were used to determine the volatile components in the flowers of Paulownia fortunei,Paulownia elongate,Paulownia catalpifolia,Paulownia tomentosa and natural hybrids of Paulownia fortunei.The compositions and relative contents of volatile components among different Paulownia species were analyzed,the cluster analysis and principal component analysis were further performed.The results showed that a total of 45 volatile components were identified in the flowers of five Paulownia species,mainly including 12 terpenoids,eight alcohols,seven esters,three phenols,six aldehydes,two lignans,two benzenes,two ketones,two alkanes and one ethers.The five Paulownia species had seven volatile components in common,including one terpenoid,two alcohols,one ester,two phenols and one ketone,and the number of their unique components were nine,one,four,three and zero,respectively.The relative content of alcohol compounds were the highest among the five Paulownia species,and the relative content of 1-octen-3-ol and 3-octanol were higher among alcohols.Cluster analysis showed that P.catalpifolia and P.tomentosa had higher similarity,while the P.fortunei had lower similarity with other Paulownia species.The sum of the first principal component(PC1)and the second principal component(PC2)was 70.7%,the main contribution components of PC1 were esters,alkanes,terpenoids,ethers,ketones,alcohols and benzenes,the main contribution components of PC2 were phenols,alcohols,aldehydes,ketones,ethers,benzenes and lignans.Partial least squares discriminant analysis(PLS-DA)screened out 11 distinctive volatile components with significant effects,including 1-octen-3-ol,1,2,4-trimethoxybenzene,(Z)-anethole and so on.This study can provide reference for accurate evaluation and efficient utilization of volatile components in the flowers of different Paulownia species.