文献类型：期刊文献

英文题名：Ultrasound-laccase pre-treatment enhances agarwood essential oil extraction and bioactivity

作者：Ma, Sheng[1] Chen, Yuan[1] Yan, Tingting[1] Qin, Jiahui[1] Li, Gaiyun[1]

通信作者：Li, GY[1]

机构：[1]Chinese Acad Forestry, Res Inst Wood Ind, Beijing 100091, Peoples R China

年份：2025

卷号：295

外文期刊名：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES

收录：;EI(收录号：20250217671739);Scopus(收录号：2-s2.0-85214466376);WOS:【SCI-EXPANDED(收录号:WOS:001398073200001)】；

基金：This work was financially supported by the National Key Research and Development Program of China (2022YFD2200805) . We also thank the people who participated in this research.

语种：英文

外文关键词：Agarwood essential oil; Ultrasound-assisted laccase; Sesquiterpene oxides; Antioxidant activity; Acetylcholinesterase inhibition; alpha-Glucosidase inhibition; Sustainable extraction

摘要：Agarwood essential oil is prized for its elegant aroma and pharmacological properties; however, the traditional hydrodistillation method suffers from inefficiencies, constraining the industrial potential of agarwood. We proposed an ultrasonic-assisted laccase synergistic pretreatment technique that enhanced extraction throughput by 70.90 % compared to the traditional method by facilitating pore formation in agarwood and expediting the release of essential oil. The essential oil extracted using this method retained a similar aromatic profile to the traditional method but achieved a higher sesquiterpene oxide concentration (95.3 % vs. 89.6 %). Additionally, it exhibited notable improvements in antioxidant activity, acetylcholinesterase inhibition, and alpha-glucosidase inhibition, attributed to the increased affinity of sesquiterpene oxides for enzymes through hydrogen bonding, covalent interactions, and hydrophobic effects with amino acid residues. This approach not only maximises the efficiency of agarwood essential oil extraction and amplifies its bioactive properties, but also offers a sustainable and eco-friendly alternative, promoting the long-term vitality of the agarwood industry.